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pcDNA3.1_Hygro(+)_GRHL1-K116A-L3F6H

Plasmid

#177769

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K116A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K116A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-T139A-L3F6H

Plasmid

#177770

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (T139A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

T139A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-S374A-L3F6H

Plasmid

#177772

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (S374A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

S374A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA3.1_Hygro(+)_GRHL1-K592A-L3F6H

Plasmid

#177773

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K592A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K592A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-L77A/L80A

Plasmid

#100273

Purpose

Expresses GST-Stx3-L77A/L80A in bacteria.

Depositor

Thomas Weimbs

Publication

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-L77A/L80A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-E78A/E83A

Plasmid

#100272

Purpose

Expresses GST-Stx3-E78A/E83A in bacteria.

Depositor

Thomas Weimbs

Publication

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-E78A/E83A (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

Tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T3-Stx3-LE165/166AA

Plasmid

#100271

Purpose

Expresses GST-tagged open-conformation mutant of Stx3 in bacteria.

Depositor

Thomas Weimbs

Publication

Giovannone et al Sci Rep. 2020 Dec 7;10(1):21350. doi: 10

Insert

Stx3-1-265-LE165/166AA (Stx3 Rat)

Tags

GST

Expression

Bacterial

Mutation

No transmembrane domain; residues 4-264 present, …

Promoter

tac

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACP7-7A-3xHA_pJWM_081

Plasmid

#260158

Purpose

Intestine expressed phosphatase inactive ACP7-7A/F21A3.11(7 point mutations) with 3xHA-tags

Depositor

Anne Brunet

Publication

Miklas et al Cell Rep. 2026 Aug 27;45(9):117902. doi:

Insert

ACP7/F21A3.11-7A

Tags

3xHA Tag

Expression

Worm

Mutation

7 point mutations - D156A, D185A, Y188A, N220A, H…

Promoter

ges-1p

Available since

Sept. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pIBA2-IntHA453-SpyTag

Plasmid

#198039

Purpose

Expression plasmid for producing SpyTagged intimin

Depositor

Jack Leo

Publication

Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

Insert

eaeA

Tags

SpyTag

Expression

Bacterial

Mutation

double HA tag inserted at position 453; lacks nat…

Promoter

tet

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pICH86966::AtU6p::sgRNA:NbCysP6

Plasmid

#223217

Purpose

Expresses an sgRNA targeting the NbCysP6 gene in Nicotiana benthamiana with an Arabidopsis U6 promoter

Depositor

Tsepo Tsekoa

Publication

Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

Insert

NbCysP6 sgRNA

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Plant

Promoter

Arabidopsis U6 promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICH86966::AtU6p::sgRNA:NbVPE1a/b

Plasmid

#223218

Purpose

Expresses an sgRNA targeting the NbVPE1a/b genes in Nicotiana benthamiana with an Arabidopsis U6 promoter

Depositor

Tsepo Tsekoa

Publication

Singh et al Front Plant Sci. 2022 Aug 18;13:953654.

Insert

NbVPE1a/b

Use

CRISPR and Synthetic Biology

Tags

None

Expression

Plant

Promoter

Arabidopsis U6 promoter

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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