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pDest17_Gcn4 R2

Plasmid

#231870

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 R2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

K118R, K140R

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 L123A+

Plasmid

#231869

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 L123A+

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L123A+

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

L123A

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap2

Plasmid

#231865

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap2

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap2

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 F124I

Plasmid

#231864

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 F124I

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

F124I

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 Swap3

Plasmid

#231860

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Swap3

Depositor

Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Swap3

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pDest17_Gcn4 WT 44mer

Plasmid

#231856

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 WT 44mer

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 44mer

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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LEAPVW

Plasmid

#205671

Purpose

shRNAmir backbone for RNA interference, with Venus reporter

Depositor

Dapeng Wang

Article

Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

Insert

miR-AB

Use

Lentiviral

Promoter

EF1a

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGWB602-MpPHOT-3xFLAG

Plasmid

#228474

Purpose

MpPHOT-3xFLAG under the control of the cauliflower mosaic virus (CaMV) 35S promoter (for Agrobacterium-mediated genetic transformation.)

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

MpPHOT-3xFLAG

Expression

Bacterial and Plant

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET-MhCINS-6xHis

Plasmid

#194304

Purpose

Bacterial expression of MhCINS protein with a C terminal His-tag and transit peptide removed at N terminal

Depositor

Richard Saunders

Article

Liu et al J Ecology 2024; 00, 1–20. doi: 10.1111/1

Insert

MhCINS

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-HIS3MX6

Plasmid

#227975

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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