We narrowed to 159,481 results for: addgene

1. pMIAII-Cd4

   Plasmid

   #194401

   Purpose

   Retroviral expression of mouse CD4

   Depositor

   Michael Carroll

   Article

   Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

   Insert

   Cd4 (Cd4 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK CA RhoA

   Plasmid

   #112894

   Purpose

   promoter-inducible expression of constitutively-active RhoA

   Depositor

   Sanjay Kumar

   Article

   MacKay et al Integr Biol (Camb). 2014 Sep;6(9):885-94

   Insert

   CA RhoA (RHOA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2-HTH-HHT2

   Plasmid

   #86482

   Purpose

   yeast expression of HHT2 with His10-TEV-HA tag

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Insert

   HHT2 (HHT2 Budding Yeast)
   

   Use

   Tags

   HIS10-TEV-HA (HTH)

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pSQT1313

   Plasmid

   #53370

   Purpose

   multiplex gRNA expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   None
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-dCasRx-RBM25

   Plasmid

   #221001

   Purpose

   Transient expression of dCasRx-RBM25. EF1a promoter.

   Depositor

   Benjamin Blencowe, Mikko Taipale

   Article

   Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

   Insert

   dCasRx-RBM25
   

   Use

   CRISPR

   Tags

   NLS and NLS-HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EFS-DIO-GRAB_Ado1.0

   Plasmid

   #153291

   Purpose

   Express the adenosine sensor GRAB_Ado1.0 in Cre positive cells

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine sensor GRAB_Ado1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide Cherry

   Plasmid

   #170510

   Purpose

   Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and mCherry marker from EF-1a promoter. 3rd generation lentiviral backbone.

   Depositor

   Pavel Tolar

   Article

   Newman et al biorXiv 2021.03.02.433398

   Inserts

   S. pyogenes sgRNA cassette
   mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a and hU6

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. TNFa-GFP

   Plasmid

   #163660

   Purpose

   Expresses GFP tagged TNFa in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   tumor necrosis factor (TNF Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

   Plasmid

   #185914

   Purpose

   pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS and BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

   Promoter

   CMV and T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CRISPR_HLA_class_I_2

    Plasmid

    #164987

    Purpose

    Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA class I
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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