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pLenti-Crispr-V2-HMID.v1

Plasmid

#103062

Purpose

LentiCrisprV2 with guide targeting V1-V5 GESTALT barcodes

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

Cas9 plus expressed GESTALT V1-V5 guide

Use

Lentiviral

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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XW82

Plasmid

#65840

Purpose

MosSCI-QUAS::Δpes10::GFP

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

5XQUAS-delta-pes-10::GFP

Tags

GFP

Expression

Worm

Available Since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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AP682-1

Plasmid

#70050

Purpose

Expresses TEV::eGFP::myc::3Xflag in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

eGFP

Tags

TEV and myc::3Xflag

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pAc5 dOrai

Plasmid

#16368

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai (olf186-F Fly)

Tags

V5

Expression

Insect

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMpGWB102-Citrine-PTS1

Plasmid

#44592

Purpose

Binary vectors for expression of Citrine fluorescent protein with a peroxisome localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-PTS1

Tags

PTS1 (peroxisomal targeting signal type 1)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMT-puroR-p2a-HA-halo

Plasmid

#161979

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Article

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Insert

puroR-p2a-HA-halo-GSlinker

Available Since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCZGY2162

Plasmid

#110136

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito::GCaMP3

Tags

mito::GCaMP3

Expression

Worm

Promoter

Pcol-19

Available Since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHnCB

Plasmid

#52064

Purpose

expresses nine genes from the H. neapolitanus carboxysome operon; IPTG-inducible

Depositor

David Savage

Article

Bonacci et al Proc Natl Acad Sci U S A. 2012 Jan 10;10

Insert

CbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B

Expression

Bacterial

Available Since

April 15, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV3

Plasmid

#78631

Purpose

GESTALT V3 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V3

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP-BarcodeV4

Plasmid

#78630

Purpose

GESTALT V4 integrating barcode with Puro and EGFP

Depositor

Jay Shendure

Article

McKenna et al Science. 2016 May 26. pii: aaf7907.

Insert

GESTALT lineage tracing target V4

Use

Lentiviral

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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OMP-SpH-ACNF TV

Plasmid

#15513

Depositor

Peter Mombaerts

Article

Bozza et al Neuron. 2004 Apr 8. 42(1):9-21.

Insert

olfactory marker protein (Omp Mouse)

Use

Mouse Targeting

Mutation

OMP coding region and 3' end were replaced b…

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CONK1-lumitoxin

Plasmid

#51689

Purpose

Expression vector for CONK1-lumitoxin

Depositor

Edward Boyden

Article

Schmidt et al Nat Commun. 2014 Jan 10;5:3019. doi: 10.

Insert

CONK1-lumitoxin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mCherry-RT-mCherry

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pBY2946

Plasmid

#23223

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

SnAvi

Expression

Worm

Available Since

May 4, 2010

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pNH041

Plasmid

#42153

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

YFP

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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SaMscL (CDelta26)

Plasmid

#79027

Purpose

Staphylococcus aureus MscL with C Terminal Truncation of 26 amino acids

Depositor

Douglas Rees

Article

Liu et al Nature. 2009 Sep 3;461(7260):120-4. doi:

Insert

MscL

Tags

6x Histidine Tag

Mutation

Stop codon placed before 26 amino acids at C Term…

Promoter

T7 Promoter

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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