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The co-receptor Tspan12 directly captures Norrin to promote ligand-specific β-catenin signaling
Bruguera ES, Mahoney JP, Weis WI
bioRxiv 2024.02.03.578714 Article

Plasmids from Article

ID Plasmid Purpose
216379mFzd4CRDL(42-203)-6xHis_pAcGP67aBaculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells
216380FLAG-mFzd4-ΔC(40-513)-3C-sGFP1-10_pVL1393Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 ΔC (residues 40-513) fused to split GFP (strands 1-10)
216381hTspan12(1-305)-1D4_pTT5Mammalian expression vector to transiently express full-length human Tspan12 with a Rho1D4 tag
216382hTspan12(1-252)-6xHis-3C-sGFP11_pVL1393Baculovirus transfer vector to express human Tspan12 ∆C (residues 1-252) fused to split GFP s11
216383MBP-3C-hTspan12LEL(118-218)_pAcGP67aBaculovirus transfer vector to secrete human Tspan12 LEL (residues 118-218) fused to MBP
216384MBP-3C-hNorrin(33-133)-1D4_pAcGP67aBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218)
216385MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67aBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)

Antibodies from Article