We narrowed to 16,087 results for: grna

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pLenti SpBsmBI sgRNA Puro

Plasmid

#62207

Purpose

An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

Depositor

Rene Maehr

Article

Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only

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pUF-Cas9-pre-sgRNA

Plasmid

#137845

Purpose

Cas9 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Type

Empty backbone

Use

CRISPR

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK2/PKR_sgRNA

Plasmid

#218527

Purpose

sgRNA targeting human EIF2AK2/PKR

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK2 (EIF2AK2 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCR8-PspCas13b_gRNA[ccdbCam]_1xMS2b

Plasmid

#196847

Purpose

backbone for gRNA cloning of dpspCas13b tagged by 1xMS2

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Type

Empty backbone

Use

CRISPR

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Notch1 #1 GFP

Plasmid

#106950

Purpose

Lentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

gRNA targeting Notch1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W

Plasmid

#112925

Purpose

Lentiviral vector for SAM-CRISPRa gRNA expression with puroBFP

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

hU6 promoter, gRNA-SAM expression cassette

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1 intron 1 gRNA as2

Plasmid

#132394

Purpose

Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

gRNA targeting AAVS1 intron 1 (PPP1R12C Human)

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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7b sgRNA for EJ7-GFP reporter

Plasmid

#113624

Purpose

sgRNA/CAS9 expression plasmid to induce the 3’ double-strand break in the EJ7-GFP reporter

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7b sgRNA

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only
pHR-U6-sgRNA-CMV-puro

Plasmid

#169450

Purpose

backbone for sgRNA expression

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

sgRNA

Use

Lentiviral

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK3/PERK_sgRNA

Plasmid

#218666

Purpose

sgRNA targeting human EIF2AK3/PERK

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK3 (EIF2AK3 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX1T1 g3)-PGKpuroBFP-W

Plasmid

#211984

Purpose

Express gRNA against RUNX1T1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting RUNX1T1 (RUNX1T1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX1T1 g7-5)-PGKpuroBFP-W

Plasmid

#211985

Purpose

Express gRNA against RUNX1T1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting RUNX1T1 (RUNX1T1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human U6 promoter

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro EIF2AK1/HRI_sgRNA

Plasmid

#218529

Purpose

sgRNA targeting human EIF2AK1/HRI

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK1 (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

Plasmid

#89060

Purpose

AAV-gRNA cloning vector with GFP reporter

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Expression

Mammalian

Promoter

Chicken beta actin with partial CMV enhancer

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pAG79-attL1-pegRNA-attL2

Plasmid

#213050

Purpose

Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attL2

Depositor

Bing Yang

Article

Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

Insert

sgRNA

Expression

Bacterial

Promoter

35S-CmYCLV-AtU6

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-sp-sgRNA-RNF2_+41nick

Plasmid

#135958

Purpose

S. pyogenes sgRNA for +41 nick in RNF2

Depositor

David Liu

Article

Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

Insert

RNF2_+41 nicking sgRNA

Expression

Mammalian

Mutation

See manuscript

Promoter

U6

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1 double gRNAs

Plasmid

#162791

Purpose

Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Plasmid

#140082

Purpose

Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

Depositor

Keith Joung

Insert

pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

Use

In vitro transcription (mrna synthesis)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 17, 2020

Availability

Academic Institutions and Nonprofits only

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