We narrowed to 14,607 results for: pET

1. GB1-PRD(800-868)

   Plasmid

   #141345

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1His6-TEV-ALIX(800-868)
   

   Use

   Tags

   GB1-His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. RC(WW)-LgBit

   Plasmid

   #208931

   Purpose

   Recognition domain for in vitro MAPK-activity sensor

   Depositor

   Attila Reményi

   Article

   Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

   Insert

   WW domain of PIN1 (PIN1 Human)
   

   Use

   Tags

   HISx6, LgBit, and MBP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. eGFP-CBP

   Plasmid

   #169211

   Purpose

   Expression of eGFP-CBP protein for chitin visualization

   Depositor

   James S Fraser

   Article

   Ricardo-Gonzalez et al bioRxiv 2021.03.29.437438

   Insert

   Chitinase A1 Chitin Binding Domain
   

   Use

   Tags

   6xHis, Mxe Gyr Intein, and eGFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC_N_FEV

   Plasmid

   #167757

   Purpose

   Expresses FEV in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   FEV (FEV Human)
   

   Use

   Tags

   MAC

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMI91

   Plasmid

   #74888

   Purpose

   Shuffled 9I27 polyprotein with shuffled codons to allow facile sequencing and unique RE for the 9 cassettes for versatile cloning. See Depositor Comments for more information

   Depositor

   Piotr Marszalek

   Article

   Scholl et al Biomacromolecules. 2016 Jun 8.

   Insert

   Titin I27 x 9 (TTN Human)
   

   Use

   Tags

   His-tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSUMO-LIC-CHIKV-nsP2h

   Plasmid

   #127935

   Purpose

   Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

   Depositor

   Dahai Luo

   Article

   Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

   Insert

   CHIKV nsP2h
   

   Use

   Tags

   His6-Sumo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSP1D1R1

   Plasmid

   #97323

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional R residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1R1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of R residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPReporter

   Plasmid

   #65007

   Purpose

   E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

   Depositor

   Mattheos Koffas

   Article

   Cress et al ACS Synth Biol. 2015 Mar 30.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   none

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSP1D1G1

   Plasmid

   #97325

   Purpose

   Nanodisc scaffold protein belt. Mutant of MSP1D1 from the Sligar Lab to include an additional G residue shortly after the TEV cleavage site. Additional mass used as marker in mass spectrometry.

   Depositor

   Michael Marty

   Article

   Reid et al Anal Chem. 2017 Nov 7;89(21):11189-11192

   Insert

   MSP1D1G1
   

   Use

   Tags

   6xHis - TEV

   Expression

   Bacterial

   Mutation

   Addition of G residue after T25

   Promoter

   T7

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ALFY-FYVE 1G

    Plasmid

    #197045

    Purpose

    Expression plasmid for the FYVE domain from ALFY for recombinant expression in E. coli.

    Depositor

    Michael Ragusa

    Article

    Reinhart et al Traffic. 2021 Jan;22(1-2):23-37. doi: 10

    Insert

    ALFY (WDFY3 Human)
    

    Use

    Tags

    6xHis-GST-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. ALIX(P801G)-Strep

    Plasmid

    #180024

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ALIX(1-868, P801G)-Strep. P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    ALIX(1-868, P801G)-Strep (PDCD6IP Human)
    

    Use

    Tags

    Strep tag (WSHPQFEK)

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSD117A

    Plasmid

    #218141

    Purpose

    Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

    Depositor

    David Liu

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    SD40
    

    Use

    Tags

    AviTag-6xHis-MBP-TEV-(GGGGS)2

    Expression

    Mutation

    Promoter

    Available since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. bdSUMO-HA2-GFP-HiBit

    Plasmid

    #171017

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with HA2 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-HA2-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CAP Z alpha 1 beta 1 CP

    Plasmid

    #13451

    Depositor

    John Cooper

    Article

    Soeno et al J Muscle Res Cell Motil. 1998 Aug . 19(6

    Insert

    capping protein alpha 1 beta 1 (chicken) (CAPZB Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Dec. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

15. bdSUMO-R9-GFP-HiBit

    Plasmid

    #170990

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with R9 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-R9-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. bdSUMO-pHD118-GFP-HiBit

    Plasmid

    #171015

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHD118-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLS.546

    Plasmid

    #184963

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    priB donor G1069A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLS.540

    Plasmid

    #184959

    Purpose

    Test effect of a1/a2 length on editing fabH using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, fabH G954A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    fabH donor G954A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 2Bc-T MBP_BoMoC(ed)_I770A_6xH

    Plasmid

    #185711

    Purpose

    Recombinant B. mori truncated R2 RT expression

    Depositor

    Kathleen Collins

    Article

    Pimentel et al J Biol Chem. 2022 Mar;298(3):101624. doi

    Insert

    B. mori truncated R2 RT
    

    Use

    Tags

    6xHis and MBP

    Expression

    Bacterial

    Mutation

    Changed I770 to A

    Promoter

    T7

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. bdSUMO-pHlip-GFP-HiBit

    Plasmid

    #171016

    Purpose

    For bacterial expression of bdSUMO tagged muGFP with pHlip peptide and NanoLuc complementation peptide variant #86

    Depositor

    Angus Johnston

    Article

    Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

    Insert

    14x-His-bdSUMO-pHlip-muGFP-HiBiT
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge