We narrowed to 9,801 results for: pET

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pETM11-6xHis-TEV-hORF1p_StammerAAA

Plasmid

#202587

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A, E92A, and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Methionine 91 to Alanine, ORF1p Glu…

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETM11-6xHis-TEV-hORF1p_StammerAEA

Plasmid

#202588

Purpose

Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the M91A and L93A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification

Depositor

Liam Holt

Article

Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

Insert

ORF1p

Tags

6x His and TEV protease cleavage site

Expression

Bacterial

Mutation

Changed ORF1p Methionine 91 to Alanine, changed O…

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 sf:em-blue-shift.1

Plasmid

#191928

Purpose

Encodes a GFP design, sf:em-blue-shift.1

Depositor

Sarel Fleishman

Article

Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

Insert

sf:em-blue-shift.1

Tags

His Tag

Expression

Bacterial

Mutation

T65S Q69P S72A T108V Y145M T167V V224I

Promoter

T7

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pET24-aCatenin(82-906)-His6

Plasmid

#203332

Purpose

Bacterial expression of wild-type alpha-catenin

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2012 May 25;287(22):18492-9

Insert

CTNNA1 (CTNNA1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 82-906

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-YPet-WPRE

Plasmid

#193335

Purpose

Expresses YPet under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

YPet

Expression

Mammalian

Promoter

TRE

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED 4CA-FLAG

Plasmid

#199681

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED 4CA (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

C595.604.629.638 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED Y551A-FLAG

Plasmid

#199680

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED Y551A (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Y551 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41P-DmGW182_744-817_N

Plasmid

#147064

Purpose

Bacterial Expression of DmGW182_744-817

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmGW182_744-817 (gw Fly)

Expression

Bacterial

Mutation

one non silent mutations N799S and one silent mut…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-D603K_M

Plasmid

#146962

Purpose

Bacterial Expression of NcQde-2_506-938-D603K

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-D603K

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-K638E_M

Plasmid

#146963

Purpose

Bacterial Expression of NcQde-2_506-938-K638E

Depositor

Elisa Izaurralde

Article

Boland et al Proc Natl Acad Sci U S A. 2011 Jun 28;10

Insert

NcQde-2_506-938-K638E

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-41P-HsNot2_344-540_M

Plasmid

#146946

Purpose

Bacterial Expression of HsNot2_344-540

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_344-540 (CNOT2 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmGW182-RBD_F

Plasmid

#146290

Purpose

Bacterial Expression of DmGW182-RBD

Depositor

Elisa Izaurralde

Article

Eulalio et al Nucleic Acids Res. 2009 May;37(9):2974-8

Insert

DmGW182-RBD (gw Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmTral_1-84_C

Plasmid

#146082

Purpose

Bacterial Expression of DmTral_1-84

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmTral_1-84 (tral Fly)

Expression

Bacterial

Mutation

one silent mutation A198C compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NusA-DmEDC3_1-101_D

Plasmid

#146126

Purpose

Bacterial Expression of DmEDC3_1-101

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2007 Dec . 27(24):8600-11

Insert

DmEDC3_1-101 (Edc3 Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM41P-HsUPF3b-iso2_145-470_K

Plasmid

#146754

Purpose

Bacterial Expression of HsUPF3biso2_145-470

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3biso2_145-470 (UPF3B Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NdeI-NcQde-2_V

Plasmid

#147747

Purpose

Bacterial Expression of NcQde-2

Depositor

Elisa Izaurralde

Insert

NcQde-2

Expression

Bacterial

Mutation

1 non silent mutation P761S compared to the seque…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41)

Plasmid

#197939

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(G41S)

Plasmid

#197940

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-G41S, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_CoA-Snifit(V97T)

Plasmid

#197941

Purpose

Expression of CoA-Snifit in bacterial cells

Depositor

Kai Johnsson

Article

Xue et al Nat Chem Biol. 2022 Oct 31. doi: 10.1038

Insert

Halo-GFP-ecPanK

Expression

Bacterial

Mutation

PanK=PanK-V97T, D127A,L277W,F252Y

Available Since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

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pETM-60-NcQDE2_506-938-H609A_O

Plasmid

#147083

Purpose

Bacterial Expression of NcQde-2_506-938-H609A

Depositor

Elisa Izaurralde

Insert

NcQde-2_506-938-H609A

Expression

Bacterial

Available Since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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