We narrowed to 7,168 results for: tet

1. TriEx-4 DHX36

   Plasmid

   #68368

   Purpose

   Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

   Depositor

   James Vaughn

   Article

   Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

   Insert

   DHX36 (DHX36 Human)
   

   Use

   Tags

   Histidine

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV ie enhancer/promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234954

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

   Plasmid

   #234953

   Purpose

   Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

   Depositor

   Agnel Sfeir

   Article

   Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQCXIP mScarlet-MOSPD2 RD/LD

   Plasmid

   #186476

   Purpose

   Expression of human MOSPD2 (mutant R404D/L406D, unable to bind FFAT motifs) fused to mScarlet in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
   

   Use

   Retroviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pTRE-FLEX-EGFP-WPRE-bGHpA

   Plasmid

   #65449

   Purpose

   Can be used to generate AAV virus that will express EGFP from the tetO promoter in a Cre-dependent manner

   Depositor

   Hongkui Zeng

   Article

   Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetO

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP mScarlet-ER [TM(SAC1)]

   Plasmid

   #186572

   Purpose

   Expression of the Endoplasmic Reticulum marker mScarlet-ER [TM(SAC1)] in mammalian cells

   Depositor

   Catherine Tomasetto

   Article

   Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

   Insert

   SAC1 (SACM1L Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C-terminal transmembrane region of the protein: 5…

   Promoter

   Available since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-N2-6MYC-hMID2

   Plasmid

   #51035

   Purpose

   mammalian expression of myc-tagged human MID2

   Depositor

   Timothy Cox

   Article

   Short et al BMC Cell Biol. 2002;3:1. Epub 2002 Jan 4

   Insert

   MID2 (MID2 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCW57-Cx46-IRES-GCaMP6s

   Plasmid

   #188238

   Purpose

   3rd generation, inducible bicistronic lentiviral plasmid for expression of human connexin 46 and GCaMP6s

   Depositor

   Fabio Mammano

   Article

   Nardin et al Int J Mol Sci. 2022 Jun 30;23(13). pii:

   Insert

   GJA3 (GJA3 Human)
   

   Use

   Lentiviral

   Tags

   GCaMp6s

   Expression

   Mammalian

   Mutation

   Promoter

   Tight TRE promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.1-Kozak-SP-SNAPf-lk-EK-ADGRL3-CTF

   Plasmid

   #201217

   Purpose

   pCDNA3.1 with Kozak (GCCACC), ADGRL3 signal peptide, SNAP-tag, GS linker, and Flag-tag (EK site) ahead of ADGRL3-CTF

   Depositor

   Jonathan Javitch

   Article

   Perry-Hauser et al J Biol Chem. 2022 Dec;298(12):102594. do

   Insert

   ADGRL3 (Adgrl3 Mouse)
   

   Use

   Tags

   Flag and SNAP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(-)-C2C1-ErNaR-TS-EYFP-ES

    Plasmid

    #209851

    Purpose

    Expression of human codon-optimized light-driven sodium pump ErNaR in mammalian cells

    Depositor

    Volker Busskamp

    Article

    Podoliak et al Nat Commun. 2024 Apr 10;15(1):3119. doi:

    Insert

    light-driven sodium pump ErNaR
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL40C_PGKintron_Cas9_Green

    Plasmid

    #134966

    Purpose

    Lentiviral vector coding Cas9 and mNeonGreen

    Depositor

    Beat Bornhauser

    Article

    Huang et al Cancer Cell. 2019 Dec 9;36(6):630-644.e9

    Insert

    Cas9-P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK promoter with beta-globin intron

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQCXIP mScarlet-MOSPD2 W201E

    Plasmid

    #186477

    Purpose

    Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to mScarlet in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Use

    Retroviral

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRLNeo-pEF1a-p53ashL344P-mCerulean

    Plasmid

    #69581

    Purpose

    Expresses p53-L344P mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344P (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344P mutation that prevents oliogomerization…

    Promoter

    EF1alpha

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. human rSHIP2 (18-893)

    Plasmid

    #128568

    Purpose

    Expression of human SHIP2 in bacteria

    Depositor

    Christophe Erneux

    Article

    Pesesse et al FEBS Lett. 1998 Oct 23;437(3):301-3.

    Insert

    SHIP2 (INPPL1 Human)
    

    Use

    Tags

    His Xpress (N-terminal)

    Expression

    Bacterial

    Mutation

    SHIP2 coding sequence from amino acid 18 (A) till…

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)

    Plasmid

    #36881

    Depositor

    Liqun Luo

    Article

    Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

    Inserts

    GFP
    tTA2
    beta-globin intron
    Neo
    tdT-3Myc
    insulator
    

    Use

    Tags

    3 Myc tags

    Expression

    Mammalian

    Mutation

    deleted nucleotides after nucleotide 274, inserti…

    Promoter

    CAG (chicken beta actin promoter and CMV enhancer…

    Available since

    Aug. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRLNeo-pEF1a-p53ash-mKate2-splitmVenusN

    Plasmid

    #69582

    Purpose

    Expresses p53 tagged with mKate2 and split N-term mVenus

    Depositor

    Galit Lahav

    Article

    Gaglia et al Mol Syst Biol. 2014 Oct 24;10:753. doi:

    Insert

    p53 (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mKate2 and split mVenus - N term

    Expression

    Mammalian

    Mutation

    7 silent mutations in p53 DNA to prevent targetin…

    Promoter

    EF1alpha

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Strep-His-Tev-hCGNL1

    Plasmid

    #236520

    Purpose

    For expression of Paracingulin (human) in insect cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (CGNL1 Human)
    

    Use

    Tags

    2xStrep-10xHis-Tev

    Expression

    Insect

    Mutation

    WT

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-cCGNL1-Myc

    Plasmid

    #236516

    Purpose

    For expression of Paracingulin (dog) in mammalian cells

    Depositor

    Sandra Citi

    Article

    Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

    Insert

    CGNL1 (CGNL1 Canis lupus familiaris (dog))
    

    Use

    Tags

    GFP and Myc

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. EW482 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) [D241C, M243Q] (FLP-IN)

    Plasmid

    #236128

    Purpose

    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing and function-restoring mutations under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A in ADAR2dd; V241C, H243Q in PUF-9R

    Promoter

    CMV promoter with two TetR binding sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. EW267 CMV-TO-ADAR2dd(E488Q, T501A)-PUF-9R-MARIAcomp(V241D, H243M) (FLP-IN)

    Plasmid

    #236127

    Purpose

    Plasmid encoding ADAR2dd attached to PUF-9R with deimmunizing mutations under control of CMV promoter with two TetR binding sites

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    ADAR2dd-PUF-9R (ADARB1 Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A in ADAR2dd; V241D, H243M in PUF-9R

    Promoter

    CMV promoter with two TetR binding sites

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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