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Halo-SHLD1 HRD

Plasmid

#207076

Purpose

Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag flanked by human SHLD1 locus sequences (SHLD1 Human, Synthetic)

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME nls EGFP WPRE bGH pA (JDW 488)

Plasmid

#224540

Purpose

A Gateway compatible middle entry clone containing a 3x-NLS-EGFP for labeling the nucleus

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

nls-EGFP-WPRE-bGH-pA

Use

Gateway Cloning

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pME-LexA-mODC-LexOP-35SCaMV (JDW 1348)

Plasmid

#224505

Purpose

Gateway compatible middle entry clone containing a Destablized LexA and LexOperon with minimal CaMV 35S promoter as an all in one casette for RU486 driven gene expression

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

LexA-mODC-LexOP-35SCaMV

Use

Gateway Cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-sfGFP(158-238)

Plasmid

#220744

Purpose

GAL4-sfGFP(158-238) construct expression in mammlian cells.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

GAL4-sfGFP(158-238)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG16

Plasmid

#220749

Purpose

NIR-FbLAG16 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG16

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-NIR-FbLAG30

Plasmid

#220750

Purpose

NIR-FbLAG30 expression in mammalian cells. pAAV vector.

Depositor

Vladislav Verkhusha

Article

Barykina et al Nat Commun. 2024 Sep 6;15(1):7788. doi:

Insert

NIR-FbLAG30

Use

AAV and Affinity Reagent/ Antibody

Promoter

CAG

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-SHLD2 HRD

Plasmid

#207078

Purpose

Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous SHLD2 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag with internal PuroR cassette flanked by human SHLD2 locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPAP_scFv

Plasmid

#183733

Purpose

Expresses anti-lysozyme scFv antibody

Depositor

Jun Ishii

Article

Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

Insert

Anti-lysozyme scFv

Tags

His6-tag

Expression

Yeast

Promoter

AOX1 promoter

Available Since

Feb. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

Plasmid

#194973

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

Plasmid

#194975

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mEGFP-Gphn_P1

Plasmid

#194978

Purpose

AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)

Use

AAV

Tags

mEGFP

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_P1

Plasmid

#194972

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

Plasmid

#194974

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 Q770A E774K

Plasmid

#175770

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 Q770A E774K

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

Q770A E774K

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCD86-mEYFP(Q69K)

Plasmid

#190746

Purpose

Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al Biophys J. 2025 Oct 21;124(20):3354-3365

Insert

CD86 (CD86 Human)

Tags

mEYFP(Q69K)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1 (M50P)

Plasmid

#178651

Purpose

Mammalian expression of myc-tagged MR1 (M50P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

M50P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (H83P)

Plasmid

#178652

Purpose

Mammalian expression of myc-tagged MR1 (H83P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

H83P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4 MR1 (Q153P)

Plasmid

#178653

Purpose

Mammalian expression of myc-tagged MR1 (Q153P)

Depositor

Erik Procko

Article

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Mutation

Q153P

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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OTUB1/pRetroX-IRES-DsRed#10

Plasmid

#180279

Purpose

Plasmid encoding the human protease OTUB1 without a tag. Polycistronic red fluorescent retroviral vector (unmodified from TAKARA BIO).

Depositor

Andre Catic

Article

Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

Insert

human Otubain-1 (OTUB1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 632-639D

Plasmid

#175771

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 632-639D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Mutation

632-639D

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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