We narrowed to 616 results for: LC3

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Aquamarine-LC3B G120A-TdLanYFP

Plasmid

#228561

Purpose

Encodes an uncleavable version of the LC3B biosensor. Mutated LC3B is flanked by the Aquamarine/TdLanYFP donor/accpetor FRET pair and under the control of the CMV promoter

Depositor

Giulia Bertolin

Publication

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

Aquamarine and TdLanYFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-LC3B HRD

Plasmid

#207551

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-LC3B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLJM60-FLAG-SLC38A9.1 H60A

Plasmid

#71863

Purpose

lentiviral stable expression

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Histidine 60 mutated to Alanine

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60-FLAG-SLC38A9.1 L74A

Plasmid

#71866

Purpose

lentiviral stable expression

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Leucine 74 mutated to Alanine

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pLJM60-FLAG-SLC38A9.1 P90A

Plasmid

#71867

Purpose

lentiviral stable expression

Depositor

David Sabatini

Publication

Wang et al Science. 2015 Jan 9;347(6218):188-94. do

Insert

SLC38A9.1 (SLC38A9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized, Proline 90 mutated to Alanine

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223 ATG3 C264A

Plasmid

#129293

Purpose

Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed Cysteine 264 to Alanine

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 ATG3 K243R

Plasmid

#129294

Purpose

Gateway entry clone encoding human ATG3 K243R

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Gateway Cloning

Mutation

changed Lysine 243 to Arginine

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 K295R

Plasmid

#129302

Purpose

Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

Depositor

Robin Ketteler

Publication

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Lysine 295 to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN GBRP

Plasmid

#89293

Purpose

For expression of the mammalian Atg8 protein GABARAP without a tag

Depositor

Michael Lazarou

Publication

Nguyen et al J Cell Biol. 2016 Dec 19;215(6):857-874.

Insert

GABARAP (GABARAP Human)

Use

Retroviral

Expression

Mammalian

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SLC38A1-GW-NL-myc

Plasmid

#258342

Purpose

Myc-nanoluciferase fusion protein used in luciferase immunoprecipitation system (LIPS) autoantibody validation assays

Depositor

Christopher Bartley

Insert

SLC83A1 (Solute carrier Family 38 Member 1) (SLC38A1 Human)

Tags

myc-nanoluciferase

Expression

Mammalian

Available since

Aug. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti CRISPRv2 sgSLC38A2#1

Plasmid

#237230

Purpose

Plasmid for SNAT2 (SLC38A2) CRISPR knockout in human cell lines.

Depositor

Seth Parker

Publication

Koe et al Am J Physiol Cell Physiol. 2026 Jun 29.

Insert

Inserted sgRNA targetting SLC38A2 (SLC38A2 Human, Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available since

July 1, 2026

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_GST-LC3C

Plasmid

#223727

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Publication

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

LC3C (MAP1LC3C Human)

Tags

GST

Expression

Bacterial

Mutation

aa 2-126 only, with Glycine exposed

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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Aquamarine-LC3B G120A

Plasmid

#228563

Purpose

Encodes a donor-only, Aquamarine-tagged version of the inactive LC3B biosensor under the control of the CMV promoter

Depositor

Giulia Bertolin

Publication

Gokerkucuk et al Autophagy. 2023 Aug;19(8):2275-2295. doi

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

Aquamarine

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-LC3B HRD

Plasmid

#207539

Purpose

Homologous recombination donor to integrate a 3xFLAG-HaloTag at the endogenous LC3B locus in human cells

Depositor

Jens Schmidt

Publication

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

HaloTag with internal PuroR cassette flanked by human LC3B locus sequences

Tags

HaloTag

Expression

Mammalian

Promoter

Endogenous

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAsh-MCL-LC3B

Plasmid

#204573

Purpose

Expresses Ash-tagged LC3B in mammalian cells

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

LC3B (MAP1LC3B Human)

Expression

Mammalian

Mutation

delta121-125aa

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TalO8-GLC3-CFP

Plasmid

#178307

Purpose

CoTrIP plasmid with GLC3 promoter driving CFP's expression

Depositor

Brian Zid

Publication

Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

Insert

Glc3pr-CFP-tAdh1

Expression

Yeast

Promoter

GLC3 and NA

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP.N3-myc-SLC35A2

Plasmid

#186285

Purpose

transient expression of N-terminal myc tagged SLC35A2 isoform c/UGT1 in mammalian cells

Depositor

Somshuvra Mukhopadhyay

Publication

Li et al Glycobiology. 2019 Jun 1;29(6):490-503.

Insert

SLC35A2 (SLC35A2 Human)

Tags

MYC

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc110

Plasmid

#171893

Purpose

Promoter SPLc3 and RBSc110 in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc110

Use

Synthetic Biology

Mutation

N/A

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc249

Plasmid

#171903

Purpose

Promoter SPLc3 and RBSc249 in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc249

Use

Synthetic Biology

Mutation

N/A

Available since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pStA0::SPLc3-RBSc21

Plasmid

#171884

Purpose

Promoter SPLc3 and RBSc21 in pStA0

Depositor

John Heap

Publication

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Promoter SPLc3 and RBSc21

Use

Synthetic Biology

Mutation

N/A

Available since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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