We narrowed to 5,993 results for: pCas
-
Plasmid#119768PurposeConversion of C to T in wheat plants or monocotyledons protoplastsDepositorInsertAPOBEC3A-nCas9-NLS-UGI-NLS
TagsNLS (flanking UGI)ExpressionPlantMutationnCas9 has a mutation D10A and E945K in wild type …Promotermaize Ubiquitin-1Available SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMCS-rybozyme-IRES-CAS9
Plasmid#64668PurposePlasmid encoding multiple cloning site, rybozyme and IRES-CAS9.DepositorInsertsCAS9
HDV ribozyme
UseCRISPRTagsFlag, Internal ribosome entry site, and guide RNA…ExpressionMammalianAvailable SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pREDIT_Cas9n-MS2-BB_BbsI
Plasmid#164804PurposeREDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFPDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTrichoGate-1
Plasmid#160701Purposederivative vector of pCAMBIA1380 containing lacZ bordered by BsaI restriction sitesDepositorInsertlacZ
UseSynthetic BiologyMutationLacZ bordered by BsaI restrction sitesAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABE8e-dimer
Plasmid#138490PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
SPDK3860
Plasmid#199245PurposeModified Tobacco rattle virus (TRV) RNA2 vector for editing of Nicotiana benthamiana Phytoene desaturase (PDS) geneDepositorInsertModified TRV RNA2 with PEBV promoter and AtIleu-tRNA mobile RNA sequence
UseT-dna vectorPromoterpPEBVAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
ePPEmax*
Plasmid#205244PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
CAG-Cremyc-2A-GFP
Plasmid#116879PurposeExpress Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.DepositorInsertCremyc-2A-GFP
ExpressionMammalianAvailable SinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPPEM
Plasmid#162467PurposeDestination Expression vector for monocot plant primer editingDepositorInsertCas9H840A
TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromotermaize UbiAvailable SinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Amp_CRISPIE donor B2
Plasmid#172843PurposeCRISPIE donor B2 (Zhong et al, eLife 2021), mRuby3 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 or DRS-2 sgRNA (with SpCas9).DepositorInsertCRISPIE designer exon (phase 0-0) encoding mRuby3
UseCRISPRAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Double UP mNeon to mScarlet Rac1-V12
Plasmid#125136PurposeNatively produces mNeonGreen, in the presence of Cre will be recombined to produce mScarlet and an untagged Rac1-V12 (Constitutively Active).DepositorInsertmNeon/mScarletP2A Rac1-V12
ExpressionMammalianMutationRemoved Not1 site in mScarlet. 12th AA in Rac1 ch…PromoterCAGAvailable SinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
NG-ABE8-dimer
Plasmid#138497PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (NG)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXYB089
Plasmid#239628PurposePlant RNA Vision constructs with no gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01
stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyExpressionPlantAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB082
Plasmid#239578PurposePlant RNA Vision constructs with 41 bp gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01-gRNA01
gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyExpressionPlantAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB083
Plasmid#239579PurposePlant RNA Vision constructs with 82 bp gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01-gRNA03
gRNA04-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyExpressionPlantAvailable SinceJune 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pX330-PITCh-PXN
Plasmid#227315PurposeExpresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in.DepositorAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMH-Cas9-gate
Plasmid#113742PurposeGateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.DepositorInsertCas9
UseCRISPR; Gateway destination vectorPromoterZ. Mays ubiquitin promoter, 35S promoterAvailable SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXEE401B
Plasmid#91716PurposeCRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT25
Plasmid#223397PurposeT-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.DepositorInsertAtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
TDP43 NOTAG2
Plasmid#28207DepositorAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only