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lvl2-[landingPad-backbone]

Plasmid

#229210

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A1_M13ps

Plasmid

#231414

Purpose

M13 phagemid that expresses sgRNA-A1 from a strong constitutive promoter. (This plasmid is a gift of the Jaramillo Lab, where it is called PAJ164.)

Depositor

Alfonso Jaramillo

Article

Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

Insert

sgRNA-A1

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMaCTag-31

Plasmid

#120010

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PP310

Plasmid

#78979

Purpose

245R. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP362

Plasmid

#78981

Purpose

255R. Promoter: scTEF1, minimal Promoter: GAP. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP311

Plasmid

#78980

Purpose

246R. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses GFP and RFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

GFP
RFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP326

Plasmid

#78983

Purpose

245B. Promoter: GAP, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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(P6)RBSS6

Plasmid

#251391

Purpose

Synthetic RBS used in expression in Synechocystis

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S6

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P2)RBSS6

Plasmid

#251371

Purpose

Synthetic RBS used in expression in Synechocystis

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S6

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P4)RBSS6

Plasmid

#251381

Purpose

Synthetic RBS used in expression in Synechocystis

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS S6

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P4)RBSB

Plasmid

#251383

Purpose

RBS designed in silico [75] for high expression in E. coli

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS B

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P2)RBSB

Plasmid

#251373

Purpose

RBS designed in silico [75] for high expression in E. coli

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS B

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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(P6)RBSB

Plasmid

#251393

Purpose

RBS designed in silico [75] for high expression in E. coli

Depositor

Kate Adamala, Pauli Kallio

Article

Venero et al BMC Biotechnol. 2026 Feb 16;26(1):38. do

Insert

RBS B

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_383

Plasmid

#250618

Purpose

Himar.001 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Article

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.001

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_384

Plasmid

#250619

Purpose

Himar.003 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Article

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.003

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_385

Plasmid

#250620

Purpose

Himar.004 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Article

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.004

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_386

Plasmid

#250621

Purpose

Himar.005 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Article

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.005

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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pGLU_387

Plasmid

#250622

Purpose

Himar.007 Promoter Destination Vector [ERM] [Barcoded]

Depositor

Nili Ostrov

Article

Ortiz et al bioRxiv 2025.12.05.692644

Insert

Himar.007

Use

Synthetic Biology

Expression

Bacterial

Available Since

March 23, 2026

Availability

Academic Institutions and Nonprofits only

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lvl2-[lenti-backbone]

Plasmid

#229208

Purpose

lvl 1 backbone

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-I-E1

Plasmid

#229161

Purpose

lvl 0 insulator

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

E1

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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