We narrowed to 2,914 results for: GRI;

1. p3xFLAG SARS-CoV2 RdRp.co

   Plasmid

   #154008

   Purpose

   Expression of codon-optimized SARS-CoV2 Nsp12 (RdRp) in mammalian cells

   Depositor

   Patrick Moore

   Article

   Meng et al J Med Virol. 2023 Jan;95(1):e28246. doi:

   Insert

   Codon-optimized SARS-CoV2 Nsp12 from ORF1ab (ORF1ab Synthetic)
   

   Use

   Tags

   3xFLAG tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 23, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pAcBac1-Mb-sfGFP 150TAG

   Plasmid

   #197570

   Purpose

   Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pscALPSpuro-HsACE2 (human)

   Plasmid

   #158081

   Purpose

   Expresses human ACE2 in mammalian cells

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   Human ACE2 (ACE2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. eGFP-proα2(I)-G610C

   Plasmid

   #119827

   Purpose

   Expresses mouse Type I procollagen α2 chain (Col1a2) with Gly610Cys mutation and GFP between signal sequence and exon 6

   Depositor

   Sergey Leikin

   Article

   Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

   Insert

   Type I procollagen α2 chain (Col1a2 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Col1a2 exons 2-5 replaced by fluorescent tag, Gly…

   Promoter

   CMV

   Available since

   Jan. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. LMP AMPK alpha1 hp1

   Plasmid

   #89489

   Purpose

   shRNA against AMPK alpha1 in mouse/human to knock down protein expression

   Depositor

   Russell Jones

   Article

   Faubert et al Cell Metab. 2013 Jan 8;17(1):113-24. doi

   Insert

   hairpin against mouse/human AMPKalpha1 (PRKAA1, Prkaa1 Human, Mouse)
   

   Use

   Mouse Targeting and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (The GFP-Puro is from a PGK promoter; the shRN…

   Available since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

6. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

   Plasmid

   #99277

   Purpose

   Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mOrange2-IRES-EGFP
   Puro
   

   Use

   Lentiviral

   Tags

   EGFP, H2B, IRES, PGK, and mOrange2

   Expression

   Mutation

   Promoter

   EF1 alpha-human and PGK-mouse

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMS-dsRed2-WT LMNA-GFP minigene

   Plasmid

   #128230

   Purpose

   Lamin A splicing reporter

   Depositor

   Tom Misteli

   Article

   Shilo et al Nucleic Acids Res. 2019 Apr 22. pii: 547

   Insert

   lamin A (LMNA Human)
   

   Use

   Tags

   GFP and SV40-driven dsRed2 to identify transfecte…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS D614G soluble Spike

   Plasmid

   #164651

   Purpose

   Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 soluble spike (S SARS-CoV-2)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   D614G

   Promoter

   CAG

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

9. alpha actinin delta ABD-GFP

   Plasmid

   #66935

   Purpose

   Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

   Depositor

   Michael Sheetz, Pere Roca-Cusachs

   Article

   Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Alpha-actinin 1 (ACTN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   actin binding domain (amino acids 30–253) is dele…

   Promoter

   CMV

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA5 CMV 3xFG eIF4G1 +MIC

    Plasmid

    #158776

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform including the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1 (with microexon)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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