We narrowed to 42,665 results for: cha

1. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A/pET28HMT

   Plasmid

   #111398

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D/pET28HMT

   Plasmid

   #111399

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP Tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/I346D

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L536A/pET28HMT

   Plasmid

   #111400

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/S536A/L540A

   Promoter

   T7

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D/pET28HMT

   Plasmid

   #111451

   Purpose

   Protein expression in E.Coli

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   His Tag and MBP tag

   Expression

   Bacterial

   Mutation

   Kv7.4 AD (325-645, C643A, Δ 368-492)/S536D/L540D

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. PX458-3xHA-FnCas9

   Plasmid

   #130969

   Purpose

   3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

   Insert

   FnCas9
   

   Use

   CRISPR

   Tags

   3xHA-NLS and NLS-T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. PX458-3xHA-en31FnCas9ABEmax8.17d

   Plasmid

   #201956

   Purpose

   Mammalian expression plasmid of en31FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Debojyoti Chakraborty

   Article

   Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

   Insert

   bpNLS-en31FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDF-His-mTET1CD∆cat

   Plasmid

   #81054

   Purpose

   bacterial expression of catalytic dead catalytic domain of murine TET1

   Depositor

   Primo Schaer

   Article

   Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

   Insert

   TET1 (Tet1 Mouse)
   

   Use

   Tags

   6HIS

   Expression

   Bacterial

   Mutation

   catalytic domain amino acid 1367-2057 with histid…

   Promoter

   T7

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

   Plasmid

   #198569

   Purpose

   SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector

   Depositor

   Monther Abu-Remaileh

   Article

   Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

   Insert

   SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
   

   Use

   Retroviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   changed glutamic acid E164 to lysine (E164K)

   Promoter

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458-3xHA-SpCas9

    Plasmid

    #130968

    Purpose

    3xHA tagged Cas9 from S. pyogenes with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    3xHA-NLS-SpCas9
    

    Use

    CRISPR

    Tags

    3x HA, EGFP, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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