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pWZ157

Plasmid

#163639

Purpose

pcn-1>PCN-1::GFP expression plasmid for CRISPR/Cas9 integration at ttTi4348 site on chromosome I

Depositor

David Matus

Publication

Adikes et al Elife. 2020 Dec 22;9. pii: 63265. doi: 1

Insert

pcn-1 (pcn-1 Nematode)

Use

CRISPR

Tags

GFP

Expression

Worm

Promoter

pcn-1

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDC24

Plasmid

#131368

Purpose

expression of MEG-3Cterm (aa545-862) in C. elegans

Depositor

Geraldine Seydoux

Publication

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa545-862) (meg-3 Nematode)

Tags

His

Expression

Bacterial

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pDC21

Plasmid

#131367

Purpose

expression of MEG-3IDR (aa1-544) in C. elegans

Depositor

Geraldine Seydoux

Publication

Smith et al Elife. 2016 Dec 3;5. pii: 21337. doi: 10

Insert

meg-3 (aa1-544) (meg-3 Nematode)

Tags

His

Expression

Bacterial

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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MPS-1-cmyc

Plasmid

#113059

Purpose

Expresses worm Myc-tagged MPS-1 in mammalian cells

Depositor

Federico Sesti

Publication

Wang et al Biophys J. 2007 Nov 1;93(9):3083-91. doi

Insert

MiRP K+ channel accessory Subunit 1 (mps-1 Nematode)

Tags

c-Myc

Expression

Mammalian

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDP-let-7_wt

Plasmid

#26558

Depositor

Chang-Zheng Chen

Publication

Trujillo et al EMBO J. 2010 Oct 6;29(19):3272-85. doi:

Insert

cel-pre-let-7

Use

Transcription vector

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only

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pRSET-Cm-CspA1

Plasmid

#215398

Purpose

Cm NCPPB382 cspA1 codon-optimized for E. coli recombinant protein expression (6xHis-CspA1)

Depositor

Gitta Coaker

Publication

Stevens et al Proc Natl Acad Sci U S A. 2024 Jun 4;121

Insert

cspA1

Tags

6xHis Tag

Expression

Bacterial

Mutation

Codon-optimized for E. coli BL21(DE3)

Promoter

T7 promoter

Available since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJH2028

Plasmid

#179771

Purpose

Pnlf-1::NLF::2XGFP unc-54 3'UTR C.elegans neural expression of NLF::2XGFP

Depositor

Mei Zhen

Publication

Xie et al Neuron. 2013 Mar 20;77(6):1069-82. doi:

Insert

NLF-1::2XGFP (nlf-1 Nematode)

Tags

2XGFP

Expression

Worm

Promoter

nlf-1

Available since

March 14, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDK540

Plasmid

#134201

Purpose

Bacterial expression of C. elegans KNL1

Depositor

Iain Cheeseman

Publication

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 FL with YA mutant (knl-1 Nematode)

Expression

Bacterial

Mutation

YA

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDK134

Plasmid

#134187

Purpose

Expression of C. elegans KNL1

Depositor

Iain Cheeseman

Publication

Kern et al Mol Biol Cell. 2015 Jan 15;26(2):229-37.

Insert

KNL1 aa 69 - 479 (knl-1 Nematode)

Expression

Bacterial

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEG196

Plasmid

#40074

Depositor

Geraldine Seydoux

Publication

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (mex-5 Nematode)

Tags

MBP

Expression

Bacterial

Promoter

P-lac

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pRK015

Plasmid

#250134

Purpose

Expression of full-length PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(FL) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK049

Plasmid

#250135

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK050

Plasmid

#250136

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK011

Plasmid

#250137

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK036

Plasmid

#250138

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pNMSB88

Plasmid

#199321

Purpose

Combine with Gal4 to direct TeNL expression

Depositor

Michael Krieg

Publication

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::TeNL::let-858 3'UTR

Expression

Worm

Mutation

KD for calcium is 250 nM, 1 synthetic intron

Promoter

15xUAS::delta pes-10

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pNMSB109

Plasmid

#199322

Purpose

Combine with Gal4 to direct CaMBI 300 expression

Depositor

Michael Krieg

Publication

Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

Insert

15xUAS::delta pes-10::::CaMBI::let-858 3'UTR

Expression

Worm

Mutation

Orange CaMBI with 300 nM KD for calcium

Promoter

15xUAS:::delta pes-10

Available since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-V5-SPR

Plasmid

#127236

Purpose

Eukaryotic expression of SPR

Depositor

Kai Johnsson

Publication

Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

Insert

SPR (SPR Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

June 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::mOrange

Plasmid

#124346

Purpose

Nuclear Localized Pan-Neuronal mOrange

Depositor

Andrew Leifer

Insert

rab-3, mOrange (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZC_CeTRIC-B2_Del

Plasmid

#87345

Purpose

Inducible expression of CeTRIC-B2 in P.Pichia strains

Depositor

Zhenfeng Liu

Publication

Yang et al Nature. 2016 Oct 27;538(7626):537-541. d

Insert

TRIC-B2

Tags

polyhistidine

Expression

Yeast

Mutation

Δ253-313

Promoter

AOX1

Available since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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