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cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a)
(Plasmid #215436)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 215436 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6612
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human cAMP-dependent protein kinase type I-alpha subunit (PRKAR1A) mRNA, complete cds, C-terminal 10X-His.Thrombin Tag
  • Alt name
    R1a
  • Alt name
    PKA regulatory 1a subunit
  • Alt name
    PRKAR1
  • Species
    H. sapiens (human)
  • Mutation
    Codon Optimized for bacterial expression, T7 gene10 leader peptide for expression
  • GenBank ID
    M33336.1
  • Promoter T7 Promotor
  • Tag / Fusion Protein
    • 10X His-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    was synthesized for us by a commercial source (Genscript)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    cAMP-dependent protein kinase (PKA) regulatory 1a subunit (R1a) was a gift from James Inglese (Addgene plasmid # 215436 ; http://n2t.net/addgene:215436 ; RRID:Addgene_215436)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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