We narrowed to 6,132 results for: pCas

Showing: 5061 - 5080 of 6132 results

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MMW948: CAG-DmrC-NLS-3xFLAG-VPR

Plasmid

#104565

Purpose

Mammalian expression vector for DmrC fused to VPR activator

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

DmrC-VPR

Tags

3x FLAG and NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-eCas9-sgNT

Plasmid

#140238

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNA

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

non-targeting sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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TC1332

Plasmid

#164874

Purpose

CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQAT17

Plasmid

#223389

Purpose

T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NGG PAM; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-hA3A-Y130F-SpCas9-D10A-UGI-OsU3-gRNA scaffold

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pH-mono-MAD7

Plasmid

#170134

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al J Genet Genomics. 2021 May 3. pii: S1673

Insert

MAD7(ErCas12a)

Use

CRISPR

Expression

Plant

Promoter

maize Ubiquitin-1, switchgrass Ubiquitin-1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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SETD2(SET)-Cas9

Plasmid

#186700

Purpose

Plasmid encoding SETD2-Cas9 fusion under CMV promoter

Depositor

Jennifer Doudna

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

Insert

SETD2(SET)-Cas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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TC1681

Plasmid

#164872

Purpose

CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

Depositor

Tian Chi

Article

Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

Insert

hAPOBEC3A fused to PspCas13b (APOBEC3A )

Use

CRISPR

Expression

Mammalian

Mutation

human apobec Y132D

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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YE2-BE4max

Plasmid

#138156

Purpose

C-to-T base editor

Depositor

David Liu

Article

Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

Insert

rAPOBEC1(YE2)-nSpCas9-UGI-UGI

Tags

NLS

Expression

Mammalian

Mutation

Within rAPOBEC1 W90Y + R132E; within Cas9 D10A

Promoter

CMV

Available Since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAdEasy Flag GCN5

Plasmid

#14106

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

E189G and K195N

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

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gRNA-HEK3-Puro

Plasmid

#136282

Purpose

Expresses a guide RNA targeting HEK3 site

Depositor

Zhaohui Ye

Article

McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

Insert

sgRNA-HEK3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pRS415_pGal-nCas9(D10A)

Plasmid

#79618

Purpose

Expresses nCas9(D10A) in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Insert

SpCas9

Expression

Yeast

Mutation

D10A for nickase Cas9

Promoter

pGal1

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B1

Plasmid

#172842

Purpose

CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TGM2

Plasmid

#69239

Purpose

U6 driven SpCas9 sgRNA expression for TGM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TGM2 sgRNA (TGM2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pREDIT_Cas9-MS2-BB_BbsI

Plasmid

#164802

Purpose

REDIT backbone for wildtype Cas9, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9-T2A-EBFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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CAG sHRPb-FKBP-post mGRASP

Plasmid

#73146

Purpose

Small fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.

Depositor

Alice Ting

Article

Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

Insert

sHRPb-FKBP-post mGRASP

Tags

HA tag

Expression

Mammalian

Promoter

CAG

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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pMCS-rybozyme-IRES-CAS9

Plasmid

#64668

Purpose

Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9.

Depositor

Wataru Fujii

Article

Yoshioka et al Sci Rep. 2015 Dec 16;5:18341. doi: 10.10

Inserts

CAS9
HDV ribozyme

Use

CRISPR

Tags

Flag, Internal ribosome entry site, and guide RNA…

Expression

Mammalian

Available Since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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A3A-PBE

Plasmid

#119768

Purpose

Conversion of C to T in wheat plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Zong et al Nat Biotechnol. 2018 Oct 1. pii: nbt.426

Insert

APOBEC3A-nCas9-NLS-UGI-NLS

Tags

NLS (flanking UGI)

Expression

Plant

Mutation

nCas9 has a mutation D10A and E945K in wild type …

Promoter

maize Ubiquitin-1

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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epi-ABEmax-NG

Plasmid

#135976

Purpose

Expresses ABEmax(with SpCas9-NG), EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

Depositor

Yongming Wang

Article

Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

Type

Empty backbone

Expression

Mammalian

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only
pREDIT_Cas9n-MS2-BB_BbsI

Plasmid

#164804

Purpose

REDIT backbone for nickase Cas9n, pU6-MS2-gRNA-backbone(BbsI)-CBH-SpCas9n(D10A)-T2A-EGFP

Depositor

Le Cong

Article

Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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