We narrowed to 6,480 results for: poly

1. pAIP4_4A02

   Plasmid

   #234194

   Purpose

   Heterologous protein expression of lytic chitin monooxygenase in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   4A02
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pFastBac HTB MBP-SARM1

   Plasmid

   #195763

   Purpose

   Purification of SARM1 from insect cells

   Depositor

   Hao Wu

   Insert

   SARM1 (SARM1 Human)
   

   Use

   Tags

   HIS-MBP-TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. C85m

   Plasmid

   #120996

   Purpose

   MoClo golden gate assembly CD part for sigmaX (Bacillus subtilis RNA polymerase involved in heat response and biofilm formation). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Sigma X (ECF20_992)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. C38m

   Plasmid

   #120971

   Purpose

   MoClo golden gate assembly CD for sigma38/RpoS (RNA polymerase active at transition from exponential phase to stationary phase). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Sigma 38 (RpoS)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. MZB211

   Plasmid

   #228494

   Purpose

   10xHis-SUMO (Smt3) tagged Ssod7-fused Pfu Polymerase with fidelity enhancing mutation (E. coli based protein expression)

   Depositor

   Richard Baker

   Insert

   pol
   

   Use

   Tags

   10xHis-SUMO(Smt3)

   Expression

   Bacterial

   Mutation

   A408S

   Promoter

   T7 Promoter

   Available since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1-Hyg-NLS.EGFP.P2A.Cherry.CAAX

   Plasmid

   #52421

   Purpose

   Expresses a polyprotein cleaved by peptide 2A into nuclear GFP and membrane located mCherry

   Depositor

   Bertrand Collet

   Insert

   Fusion NLS-EGFP-P2A-Cherry-CAAX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS2+ H2B-mTagBFP2

   Plasmid

   #99267

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein mTagBFP2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   mTagBFP2-3XHA
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOpen-T7gene5

   Plasmid

   #165534

   Purpose

   DNA Pol with high fidelity and rapid extension rate (useful in copying long stretches of DNA); Has strong 3'-5' exonuclease.

   Depositor

   FreeGenes Project, Drew Endy, Jennifer Molloy

   Insert

   T7 DNA Polymerase (unmodified)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pCS2+ H2B-Katushka2

   Plasmid

   #99266

   Purpose

   Contains a fusion of human histone H2B to fluorescent protein Katushka2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

   Depositor

   Philipp Keller

   Insert

   Katushka2-3XHA
   

   Use

   Tags

   H2B

   Expression

   Mammalian

   Mutation

   Promoter

   SP6

   Available since

   Sept. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCS2+ H2B-TagRFP-T

    Plasmid

    #99271

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein TagRFP-T in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    TagRFP-T
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCS2+ H2B-mCerulean

    Plasmid

    #99268

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mCerulean in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription

    Depositor

    Philipp Keller

    Insert

    mCerulean-3XFLAG
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2+ H2B-mRuby2

    Plasmid

    #99270

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mRuby2 in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    mRuby2-3XHA
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2+ H2B-mEmerald

    Plasmid

    #99269

    Purpose

    Contains a fusion of human histone H2B to fluorescent protein mEmerald in the pCS2+ expression vector. It has the SP6 RNA polymerase site for in vitro transcription.

    Depositor

    Philipp Keller

    Insert

    mEmerald
    

    Use

    Tags

    H2B

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    Sept. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CDKL5A-c014

    Plasmid

    #53686

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CDKL5A
    

    Use

    Baculovirus expression

    Tags

    His6-TEV

    Expression

    Mutation

    Activating mutations T169D / Y171E

    Promoter

    polyhedrin promoter

    Available since

    June 13, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pFastBac HTB His-TEV-rDPP9

    Plasmid

    #217326

    Purpose

    Purification of rat DPP9 from insect cells

    Depositor

    Yifan Wang

    Insert

    DPP9
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFB-6HZB

    Plasmid

    #218680

    Purpose

    Expression of Z-tagged proteins using Baculoviruses

    Depositor

    Sebastian Mathea

    Type

    Empty backbone

    Use

    Pfb

    Tags

    His-Z-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CDKL2A-c006

    Plasmid

    #53678

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    CDKL2A
    

    Use

    Baculovirus expression

    Tags

    His6-TEV

    Expression

    Mutation

    Activating mutations T159D / Y161E

    Promoter

    polyhedrin promoter

    Available since

    June 17, 2014

    Availability

    Academic Institutions and Nonprofits only

18. Rich-pT7

    Plasmid

    #108445

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pUC57. Xenopus globin 3UTR and 5UTR, pT7, and polyA added.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    pT7

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOpen-K12polLF (exo-)

    Plasmid

    #165522

    Purpose

    DNA pol fragment used in flourescent labelling for microarray, dA and dT tailing, and ligating DNA adapters to DNA fragments.

    Depositor

    FreeGenes Project, Drew Endy, Jennifer Molloy

    Insert

    DNA Polymerase I, Large (Klenow) Fragment (3'-5' exo-)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 21, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pFastBac1/MBP-His

    Plasmid

    #38008

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    MBP (Maltose binding protein)
    

    Use

    Baculovirus expression

    Tags

    6xHIS

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits