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Plasmid#176874PurposeEmpty backbone for Golden Gate cloning into Cermak, et al., 2017 ToolkitDepositorTypeEmpty backboneUseCloningTagsExpressionMutationPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only
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pTAG2
Plasmid#176864PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_2
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG3
Plasmid#176866PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_3
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTAG4
Plasmid#176867PurposeFirst sgRNA in a tRNA separated array of sgRNAsDepositorInsertsgRNA_4
UseCRISPRTagsExpressionMutationWTPromoterAvailable sinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos2-del6-22_X
Plasmid#147961PurposeMammalian Expression of HsNanos2-del6-22DepositorInsertHsNanos2-del6-22 (NANOS2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP225-Lenti-CMV-Flag-GluN2A-WPRE-pA
Plasmid#84274PurposepLenti vector backbone designed to express Flag-GluN2A from a CMV promoter.DepositorInsertFlag-GluN2A
UseLentiviralTagsFlagExpressionMutationPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP200-Lenti-TRE3G-Flag-GluN2A-WPRE-pA
Plasmid#164724PurposepLenti vector backbone designed to express Flag-GluN2A from a TRE3G promoter.DepositorInsertGluN2A
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP202-Lenti-TRE3G-GFPGluN2A-WPRE-pA
Plasmid#164776PurposepLenti vector backbone designed to express GFP-GluN2A from a TRE3G promoter.DepositorInsertGluN2A
UseLentiviralTagsGFPExpressionMutationPromoter1.3αCaMKIIAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP214-Lenti-0.5Synapsin P-GFP- WPRE-pA
Plasmid#62929PurposepLenti vector backbone designed to express GFP from a 0.5Synapsin promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter0.5synapsin and 1.3αCaMKIIAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP215-Lenti-0.4CaMKIIa P-GFP-WPRE-pA
Plasmid#62927PurposepLenti vector backbone designed to express GFP from a 0.4CaMKIIa promoter.DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter0.4αCaMKII and 1.3αCaMKIIAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJEP213-Lenti-1.1Synapsin P-GFP-WPRE-pA
Plasmid#62930PurposepLenti vector backbone designed to express GFP from a 1.1Synapsin promoter .DepositorInsertGreen Fluorescent protein (GFP)
UseLentiviralTagsExpressionMutationPromoter1.1Synapsin and 1.3αCaMKIIAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-CeSmg5-H116E_S
Plasmid#147458PurposeMammalian Expression of CeSmg5-H116EDepositorInsertCeSmg5-H116E (smg-5 Nematode)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG10011_C
Plasmid#146027PurposeInsect Expression of CG10011-3UTRDepositorInsertCG10011-3UTR (CG10011 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup_1-417-V5His6_C
Plasmid#146028PurposeInsect Expression of DmCup_1-417DepositorInsertDmCup_1-417 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup_419-771-V5His6_C
Plasmid#146029PurposeInsect Expression of DmCup_419-771DepositorInsertDmCup_419-771 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup-4Ala-mut_C
Plasmid#146031PurposeInsect Expression of DmCup-4Ala-mutDepositorInsertDmCup-4Ala-mut (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-V5His6_C
Plasmid#146032PurposeInsect Expression of DmCup_1-417DepositorInsertDmCup_1-417 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_419-771-V5His6_C
Plasmid#146033PurposeInsect Expression of DmCup_419-771DepositorInsertDmCup_419-771 (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGe1-V5His6_A
Plasmid#145867PurposeInsect Expression of DmGe1DepositorInsertDmGe1 (Ge-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG3077_A
Plasmid#145868PurposeInsect Expression of CG3077-3UTRDepositorInsertCG3077-3UTR (CG3077 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_1-417-Y342AL347A-V5His6_M
Plasmid#146901PurposeInsect Expression of DmCup_1-417-Y342AL347ADepositorInsertDmCup_1-417-Y342AL347A (cup Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lmajor_gp63_10_0460_D463N
Plasmid#171644PurposeExpression of L. major glcyoprotein-63 (D463N) with substrate binding site mutation from chromosome 10 in mammalian cellsDepositorInsertLmjF.10.0460 D463N
UseTagsMyc-HisExpressionMammalianMutationD463NPromoterAvailable sinceDec. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-TIR1-tTA/Zeo
Plasmid#171685Purposeconstitutive expression of TIR1-myc and tTA in sleeping beauty transposon vector with selection marker (zeocin)DepositorInsertZeocin
UseTagsExpressionMammalianMutationPromoterRPBSA promoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDmHsp70b-TACT
Plasmid#165780PurposeHSP70B promoter for fusion with bacterial promotersDepositorInsertHsp70b-TACT
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-CT-Tagging
Plasmid#165871PurposeTemplate for amplification of EGFP C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MAPRE1-Halo
Plasmid#175345PurposeProduction of HaloTag fusion proteinDepositorInsertMAPRE1 (MAPRE1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_CCDC124-Halo
Plasmid#175328PurposeProduction of HaloTag fusion proteinDepositorInsertCCDC124 (CCDC124 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3xP3
Plasmid#165779Purpose3xP3 eye driver enhancer element, requires promoter element for functionDepositorInsert3xP3
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCP6-STD
Plasmid#165795PurposeStandard grammar bacterial CP6 promoterDepositorInsertCP6 promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry TL1
Plasmid#165832PurposemCherry red fluorescent protein for N-terminal taggingDepositorInsertmCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMPRMA43
Plasmid#172944PurposepQE30 encoding Wzz mutant i191DepositorInsertShigella flexneri wzz i191 mutant gene
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CT-Tagging
Plasmid#165869PurposeTemplate for amplification of mcherry C-terminal tagging cassetteDepositorInsertVector - CT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-NT-Tagging
Plasmid#165868PurposeTemplate for amplification of EGFP N-terminal tagging cassetteDepositorInsertVector - NT Tagging
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFC31 attB O1
Plasmid#165843Purpose_C31 integrase attB site in pColE1_Omega1DepositorInsert_C31 attB
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFC31 attB O2
Plasmid#165844Purpose_C31 integrase attB site in pColE1_Omega2DepositorInsert_C31 attB
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV40L-TN
Plasmid#165827PurposeViral Simian virus 40 Late poly(a) terminator element Compatible with transposon 3' ITRsDepositorInsertSV40L
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherry TL5
Plasmid#165833PurposemCherry red fluorescent protein for C-terminal taggingDepositorInsertmCherry
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL1
Plasmid#165834PurposesfGFP green fluorescent protein for N-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP TL5
Plasmid#165835PurposesfGFP green fluorescent protein for C-terminal taggingDepositorInsertEGFP
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGGSx4 CT
Plasmid#165811PurposeFlexible peptide linker of 4 repeats of GGS, for assembly of C-terminal TL5 tags to TL4 partsDepositorInsertGGSx4 CT
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only