We narrowed to 269 results for: T7 rna polymerase
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Plasmid#175207PurposeXenopus oocyte expression vector containing YCR075C. Contains T7 RNA polymerase binding site, and adds polyA tail to YCR075C. Used for generation of mRNA, for injection into xenopus laevis oocytes.DepositorInsertYCR075C
UseMrna expression, injection into xenopus oocytesTagsPolyAPromoterT7Available SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-A114-N40
Plasmid#50561PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-A114-N40
UseCloning vectorPromoterlacAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM22082_cRed
Plasmid#117225PurposepET-28b backbone plasmid, Level 2 Golden Gate expression plasmid.DepositorInsertCanthaxanthin Operon
Tags34 amino acid N-terminal linker, Hexahistidine Ta…ExpressionBacterialMutationremoved Type IIS restriction sites within the bac…PromoterT7 RNA PolymeraseAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-BoxB-A114-N40
Plasmid#50562PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-BoxB-A114-N40
UseCloning vectorPromoterlacAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-Rluc-let-7-A114-N40
Plasmid#50563PurposeTemplate DNA for in vitro transcription using T7 polymeraseDepositorInsertLuciferase-let7-A114-N40
UseCloning vectorPromoterlacAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
Mutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDB036a
Plasmid#99215PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproD [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK and PproDAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 RRN3
Plasmid#67112PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)::NL3F10H
Plasmid#141290PurposeNLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC3F10H
UseLuciferaseTagsFlag (x3) and His (x10)ExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only