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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

Plasmid

#172916

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7s-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pyc128m

Plasmid

#158778

Purpose

soluble GCaMP expression

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6m

Use

AAV

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AiP15820 - pAAV-AiE0873m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN5820)

Plasmid

#224205

Purpose

AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14871: pAAV-AiE0779m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN4871)

Plasmid

#214865

Purpose

Expression of RiboL1-jGCaMP8s (soma targeted) in striatal direct pathway medium spiny neurons (D1 MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Has Service

AAV1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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AiP14646: pAAV-AiE0779m_6xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4646)

Plasmid

#230872

Purpose

AiE0779m_6xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal direct pathway MSNs (D1-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14133: pAAV-AiE0445h_3xC2-minBG-jGCaMP8m-WPRE3-BGHpA (Alias: CN4133)

Plasmid

#230867

Purpose

AiE0445h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8m

Use

AAV

Expression

Mammalian

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

Plasmid

#172914

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7c-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

Plasmid

#172913

Purpose

Gal4 activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7b-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Aug. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAS19

Plasmid

#158788

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans ASH neurons

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

psra-6::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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AiP15580 - pAAV-AiE0780m-minBG-axon-jGCaMP8s-WPRE3-BGHpA (Alias: CN5580)

Plasmid

#224202

Purpose

AiE0780m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal D1 MSNs

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

jGCaMP8s

Use

AAV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7b-T2A-tdTomato-p10

Plasmid

#172917

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7b-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7s-T2A-tdTomato-p10

Plasmid

#172920

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7s-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7f-T2A-tdTomato-p10

Plasmid

#172919

Purpose

LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Insert

jGCaMP7f-T2A-tdTomato

Tags

6xHis and T2A-tdTomato

Expression

Insect

Promoter

hsp70

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pJH4260

Plasmid

#191678

Purpose

Ptwk-40s-GCaMP::wScarlet unc-54 3' UTR C.elegans AVA/other neurons expression GCaMP6s wScarlet

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

GCaMP6s

Tags

wScarlet

Expression

Worm

Promoter

Ptwk-40

Available Since

Nov. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pAS16

Plasmid

#158786

Purpose

Ribosome-tagged GCaMP (soma localized) expression in C. elegans AFD neurons (and others)

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Pntc-1::GCaMP6m::rpl-1a

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHJ4

Plasmid

#158785

Purpose

Pan-neuronal nuclear-localized GCaMP expression in C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Article

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Prab-3::nls::GCaMP6m

Tags

NLS

Expression

Worm

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Salsa6f

Plasmid

#140188

Purpose

Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

Depositor

Michael Cahalan

Article

Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

Insert

Salsa6f

Tags

tdTomato-v5-GCaMP6f

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Ai38

Plasmid

#34883

Depositor

Hongkui Zeng

Article

Zariwala et al J Neurosci. 2012 Feb 29;32(9):3131-41. d

Insert

CAG-Floxed GCaMP3

Use

Mouse Targeting

Promoter

CAG

Available Since

March 7, 2012

Availability

Academic Institutions and Nonprofits only

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pGPTVII-Bar-U-MatryoshCaMP6s

Plasmid

#100024

Purpose

Plant expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Expression

Plant

Promoter

AtUBQ10

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBoBi-hLAMP2-C-GC6s

Plasmid

#154151

Purpose

To detect the calcium ion releases close to the lysosomal surface

Depositor

Sheng-cai Lin

Article

Li et al Cell Metab. 2019 Sep 3;30(3):508-524.e12

Insert

LAMP2 and GCamp6s (LAMP2 Human)

Use

Lentiviral

Mutation

WT

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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