We narrowed to 18,395 results for: Tec

1. pAF0687 Pa01_attP-mCherry donor

   Plasmid

   #193466

   Purpose

   Promoterless mcherry donor for Pa01

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01_attP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB544

   Plasmid

   #74878

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGWB505

   Plasmid

   #74847

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with sGFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPRa_VPR_yl

   Plasmid

   #107677

   Purpose

   CRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertion

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al Biotechnol J. 2018 May 5:e1700584. doi:

   Inserts

   Codon optimized dCas9-VPR
   sgRNA expression cassette
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SCR1'-tRNA and UAS1B8-TEF(136)

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB4542

   Plasmid

   #215261

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-Cas9-6xHis

   Plasmid

   #62374

   Purpose

   Expression of Cas9-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   Cas9_6xHis
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. FLHKI-pGFPN3

   Plasmid

   #21917

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Full length human HKI in pGFP-N3 (HK1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is full length human HKI in pGFP-N3

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAcHELIX-sgRNA_entry (CJT94)

   Plasmid

   #181783

   Purpose

   Expresses AcHELIX containing a nicking I-AniI fusion to AcTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-AcTnsB, AcTnsC, AcTniQ, AcCas12k
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUAS-V5-miniTurboID-NES

    Plasmid

    #116905

    Purpose

    Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SpRY-HF1-ABE8e

    Plasmid

    #185670

    Purpose

    Expresses SpRY-HF1-ABE8e in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    ecTadA(8e)-nSpRY-HF1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SpRY-HF1 D10A

    Promoter

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET29b-AK1-His

    Plasmid

    #124135

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Adenylate kinase (AK1 Chicken)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19/mChe-PM

    Plasmid

    #183168

    Purpose

    Construct for transient expression of plasma membrane-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-PM
    

    Use

    Tags

    OsRac3, O. sativa rac-like GTP-binding protein 3

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUAS-V5-TurboID-NES

    Plasmid

    #116904

    Purpose

    Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    TurboID
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGWB540

    Plasmid

    #74874

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19/NLS-mChe

    Plasmid

    #183162

    Purpose

    Construct for transient expression of cell nucleus-targeted mCherry in plants.

    Depositor

    Qinlong Zhu, Yao-Guang Liu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    NLS-mCherry
    

    Use

    Tags

    16 aa nuclear localization signal and 16 aa nucl…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Plasmid

    #92392

    Purpose

    produces AAV expressing Cal-light TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

    Insert

    TM-CaM-NES-TEV-N-AsLOV2-TEVseq-tTA
    

    Use

    AAV and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human Synapsin

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB578

    Plasmid

    #141095

    Purpose

    E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

    Depositor

    Chase Beisel

    Article

    Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

    Inserts

    Cas9 and tracrRNA
    repeat-spacer-repeat array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge