We narrowed to 9,135 results for: Pol

1. pY71-tet.I.1

   Plasmid

   #133523

   Purpose

   sfGFP driven by a T7 promoter with the tetO sequence 4 bp downstream from the promoter start site

   Depositor

   Richard Murray

   Article

   McManus et al Arch Biochem Biophys. 2019 Jul 18. pii:

   Insert

   sfGFP
   

   Tags

   Strep tag and TEV site

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFE-dabAB2 C539A

   Plasmid

   #133008

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a C539A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   C539A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFE-dabAB2 H524A

   Plasmid

   #133007

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a H524A mutation and a dabB2 gene (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   H524A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFE-dabAB2 D353A

   Plasmid

   #133006

   Purpose

   pFE carrying a dabA2 gene (Uniprot: D0KWS7) with a D353A mutation and a dabB2 gene  (Uniprot: D0KWS8)

   Depositor

   David Savage

   Article

   Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

   Inserts

   dabB2
   dabA2
   

   Expression

   Bacterial

   Mutation

   D353A

   Promoter

   tetR

   Available Since

   Nov. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b-GBdm

   Plasmid

   #129690

   Purpose

   express E. coli GreB E82C/C68S

   Depositor

   Jeff Gelles

   Article

   Tetone et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

   Insert

   GreB E82C C68S (greB E. coli)
   

   Tags

   his6

   Expression

   Bacterial

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMH-HT_SsCHAD-H29A-R32A-R36A

   Plasmid

   #127786

   Purpose

   Expresses a SsCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   mutations in His29, Arg32 and Arg36 to Ala

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMH-HT_CtCHAD-R438A-K441A

   Plasmid

   #127791

   Purpose

   Expresses a CtCHAD mutant protein in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   CtCHAD (CT0884 Chlorobium tepidum)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   Residues 208-522 (CHAD domain), mutations in Arg4…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMH-HT_ygiF-TTM

   Plasmid

   #127794

   Purpose

   Expresses the TTM/CYTH domain of ygiF in E. coli cells

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   ygiF (ygiF Escherichia coli)
   

   Tags

   6xHis tag + TEV cleavage site

   Expression

   Bacterial

   Mutation

   N-terminal TTM domain of ygiF (residues 1-200)

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-myr-Citrine-SV40pA-FKF

   Plasmid

   #119865

   Purpose

   Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   2x myristoylation

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRPB1–4stop

    Plasmid

    #112824

    Purpose

    pSMF2 with repeats 8–10 interrupted with 4 repeats containing stop codons

    Depositor

    Stephen Fuchs

    Article

    Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    pSMF2 with repeats 8–10 interrupted with 4 repeat…

    Promoter

    Native promoter

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. p5A1-T

    Plasmid

    #92017

    Purpose

    Expresses TALE targeted to lac operator with 3 TEV cut sites in its repeat domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting lac operator with 3 TEV cleavage sites
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5C3-T

    Plasmid

    #92022

    Purpose

    Expresses TALE targeted to sucA with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease

    Depositor

    Brian Pfleger

    Article

    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do

    Inserts

    TALE targeting sucA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    

    Use

    TALEN

    Tags

    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…

    Mutation

    S219V

    Available Since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAG-mPar6a K19A

    Plasmid

    #86149

    Purpose

    FLAG-mPar6a K19A - Does not bind to aPKC

    Depositor

    Senthil Muthuswamy

    Article

    Nolan et al Cancer Res. 2008 Oct 15;68(20):8201-9.

    Insert

    Par6 (Pard6a Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Lysine to Alanine mutation K19A

    Promoter

    LTR

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

14. pCellFree_G03 STAT1

    Plasmid

    #67116

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    STAT1 (STAT1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCellFree_G03 GTF2A1L

    Plasmid

    #67096

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    GTF2A1L (GTF2A1L Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pCellFree_G03 TP53

    Plasmid

    #67078

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TP53 (TP53 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCellFree_G03 IRF9

    Plasmid

    #67079

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    IRF9 (IRF9 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pCellFree_G03 E-Cad

    Plasmid

    #67046

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    E-Cad (CDH1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pCellFree_G03 TCEB2

    Plasmid

    #67049

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    TCEB2 (ELOB Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Mutation

    M6R and Q115K

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCellFree_G03 BTF3L4

    Plasmid

    #67051

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    BTF3L4 (BTF3L4 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only