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tetO-ZFPM2

Plasmid

#170707

Purpose

doxycycline-inducible overexpression of ZFPM2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

ZFPM2 (ZFPM2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

3 nonsynonymous changes: 353C>T (Ser>Phe)…

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF-G1TMSNKRS

Plasmid

#198323

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Ekanayake et al J Med Chem. 2023 Mar 15. doi: 10.1021/ac

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-Nedd1-mOrange2

Plasmid

#196860

Purpose

Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Nedd1-mOrange2 (Nedd1 Rat)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP

Plasmid

#196874

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynGFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP

Plasmid

#196876

Purpose

Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

LynEGFP

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196883

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH]

Plasmid

#196884

Purpose

Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-Lyn-GFP-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Quimeric CAG

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH]

Plasmid

#196886

Purpose

Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotransfection reporter

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

roxSTOProx-ZsGreen-bGH

Use

Cre/Lox; Dre/rox

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-AUP1 A397D

Plasmid

#185342

Purpose

Assessing G2BR function in AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 (AUP1 Human)

Tags

Flag, 6xHis

Expression

Mammalian

Mutation

A397D

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-G2BRAUP1

Plasmid

#185343

Purpose

Expression of soluble G2BR, amino acids 378-404 of human AUP1

Depositor

Allan Weissman

Article

Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

Insert

AUP1 aa 378-404 (G2BR) (AUP1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 378-404 only

Available Since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

Plasmid

#194973

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

Plasmid

#194975

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mEGFP-Gphn_P1

Plasmid

#194978

Purpose

AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)

Use

AAV

Tags

mEGFP

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_P1

Plasmid

#194972

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

Plasmid

#194974

Purpose

AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

Depositor

Günter Schwarz

Article

Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

Insert

mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)

Use

AAV

Tags

mScarlet

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph18_eGFP_CCR5TC

Plasmid

#187443

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph15_eGFP_CCR5TC

Plasmid

#187442

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_219

Plasmid

#180534

Purpose

Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_223

Plasmid

#180538

Purpose

Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-mitoX4-FKBP-HA

Plasmid

#185658

Purpose

Mitochondrial targetted (Cox8) FKBP protein with C-terminal HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

mito-FKBP

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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