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pCambia1300-NP-PTII

Plasmid

#226816

Purpose

Efficient screening of gene editing in plants (kanamycin resistance).

Depositor

Daisuke Miki

Publication

Kong et al Hortic Res. 2025 Nov 5;12(11):uhaf196. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP_delta_EHMT1

Plasmid

#228816

Purpose

Expression of human EHMT1 with deletion of region between N-terminal 300 aa and C-terminal Ank+SET

Depositor

Shravanti Rampalli

Publication

Biligiri et al PLoS Biol. 2024 Nov 7;22(11):e3002871. d

Insert

EHMT1 (EHMT1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of region between N-terminal 300 aa and …

Available since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-SilkTag-CBM-H6

Plasmid

#230903

Purpose

Bacterial expression of SilkTag

Depositor

Sesilja Aranko

Publication

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

SilkTag-CBM-H6

Tags

HisTag

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Publication

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Available since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Cterm_His

Plasmid

#220485

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_M15

Plasmid

#220488

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-bacteria_speG_E23

Plasmid

#220489

Purpose

inducible CRISPRi

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

gspeG

Use

CRISPR and RNAi

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speG_genomic

Plasmid

#220473

Purpose

heterologous expression under endogenous speG E. coli promoter

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

speG

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Nterm_His

Plasmid

#220479

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1472_Nterm_His

Plasmid

#220480

Purpose

heterologous expression of PA1472 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1472

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA1377_Nterm_His

Plasmid

#220481

Purpose

heterologous expression of PA1377 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA1377

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTP489

Plasmid

#216366

Purpose

xNup155-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup155-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC263

Plasmid

#216367

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC100

Plasmid

#216369

Purpose

xhNup155-Nb2i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC99

Plasmid

#216370

Purpose

xhNup155-Nb3i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC261

Plasmid

#216371

Purpose

xhNup155-Nb3i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-Avi-SUMOEu1

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP789

Plasmid

#216376

Purpose

xY-Nb1t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xY-Nb1t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP487

Plasmid

#216353

Purpose

xNup62-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup62-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC96

Plasmid

#216354

Purpose

xNup93-Nb1t Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb1t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP416

Plasmid

#216355

Purpose

xNup93-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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