We narrowed to 16,039 results for: GRN

1. pSK-mU6-Cj-sgRNA-SV40-puro

   Plasmid

   #192128

   Purpose

   Expresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance gene

   Depositor

   Yongming Wang

   Article

   Gao et al Mol Ther. 2023 Apr 5;31(4):1177-1187. do

   Insert

   Cj-SgRNA scaffold
   

   Expression

   Mammalian

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EGFR-L858R-tmpknot-epegRNA

   Plasmid

   #214092

   Purpose

   Lentiviral vector expressing epegRNA to induce EGFR L858R mutation

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   EGFR L858R epegRNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pXR003: CasRx gRNA cloning backbone

   Plasmid

   #109053

   Purpose

   hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

   Depositor

   Patrick Hsu

   Article

   Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDD162 (Peft-3::Cas9 + Empty sgRNA)

   Plasmid

   #47549

   Purpose

   Cas9 + sgRNA plasmid that can be modified to cleave any Cas9 target site in the C. elegans genome.

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Inserts

   Cas9
   Empty sgRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Promoter

   U6 and eef-1A.1 (eft-3)

   Available Since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti sgRNA(MS2)_puro backbone

   Plasmid

   #73795

   Purpose

   3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 and EF1A

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pC0043-PspCas13b-gRNA non target

   Plasmid

   #223698

   Purpose

   gRNA control for dPspCas13b-FTO

   Depositor

   Chuan He

   Article

   Wei et al Science. 2022 May 27;376(6596):968-973.

   Insert

   gRNA target sequence for luciferase control
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

   Plasmid

   #181745

   Purpose

   dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Insert

   human/zebrafish codon optimized SpCas9
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(SV40)-3xFLAG

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Px461-Cas9n-Trp53-sgRNA-beta

   Plasmid

   #88847

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

9. Px461-Cas9n-Trp53-sgRNA-alpha

   Plasmid

   #88846

   Purpose

   CRISPR KO of Trp53

   Depositor

   Joan Massague

   Article

   Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

   Insert

   Trp53 (Trp53 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

10. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

    Plasmid

    #183790

    Purpose

    A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

    Depositor

    Jay Shendure

    Article

    Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

    Inserts

    P2A-eGFP-TargetBC-5xTAPE-1
    pegRNA-InsertBC
    

    Use

    Synthetic Biology

    Mutation

    G>A in the gRNA scaffold- please see depositor…

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9-2A-Puro-TBK1-gRNA (PX459)

    Plasmid

    #221550

    Purpose

    Expresses Cas9 and gRNA for disruption of TBK1 gene in human cells

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    gRNA targeting human TBK1 (TBK1 Human)
    

    Expression

    Mammalian

    Promoter

    u6

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. non-targeting control gRNA (BRDN0001487130)

    Plasmid

    #80262

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. non-targeting control gRNA (BRDN0001147380)

    Plasmid

    #80225

    Purpose

    3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    Non-targeting control gRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV-U6-sgRNAfiller-PGK-Cre-EFS-mScarletSIIN

    Plasmid

    #172436

    Purpose

    Lentivirus, expresses Cre recombinase and mScarlet-SIINFEKL, with sgRNA cloning site

    Depositor

    Tyler Jacks

    Article

    Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

    Inserts

    Cre
    mScarlet-SIINFEKL(OVA257-264)+Ova 323-339
    

    Use

    CRISPR and Lentiviral

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-tmpknot-epegRNA-puro backbone

    Plasmid

    #214089

    Purpose

    Lentiviral backbone plasmid to insert epegRNAs (mpknot) of interest. The cloning site is BsmbI.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    hU6, EF1a

    Available Since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPRv2-sgRNAs-del-ZNF91-mcherry

    Plasmid

    #202822

    Purpose

    Lentiviral vector modified to express two sgRNAs that delete exon 1 within the ZNF91 gene with EF-1apha for Cas9 and mcherry expression

    Depositor

    Matthew Anderson

    Article

    Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

    Insert

    two sgRNAs that delete exon 1 within the ZNF91 gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 - two

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)

    Plasmid

    #71485

    Purpose

    Base plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon arms

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Type

    Empty backbone

    Promoter

    U6

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid

    #181792

    Purpose

    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLKO.1-puro U6 sgRNA BfuAI stuffer

    Plasmid

    #50920

    Purpose

    U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-U6-XsgRNA-ccdB-EF1a-Puromycin

    Plasmid

    #115519

    Purpose

    To construct multiple sgRNA expression vector for CRISPR

    Depositor

    Xingxu Huang

    Insert

    ccdB-sgRNA scaffold
    

    Use

    CRISPR

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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