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Plasmid#198425PurposepUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120DepositorInsertE. coli BirA
UseTagsExpressionPlantMutationPromoterpUBQ10Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFB-HTB-ASK1
Plasmid#29517DepositorInsertASK1 (SKP1 Mustard Weed)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM165/166-48nt
Plasmid#84091Purposetarget miRNA165/166 for destruction in Arabidopsis thalianaDepositorInsertSTTM165/166
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pMDC32B-AtMIR390a-NbSu-2
Plasmid#213400PurposePlant expression vector (2x35S) for expressing an amiRNA against Nicotiana benthamiana SULFUR gene from AtMIR390a precursorDepositorInsertArabidopsis MIR390a precursor including amiRNA/amiRNA* sequences for silencing N. benthamiana SULFUR gene
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGE331
Plasmid#153240PurposeM1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE342
Plasmid#153251PurposeM8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BD-cop1at(335-675)
Plasmid#44973DepositorInsertBD-COP1 (COP1 Mustard Weed)
UseTagsGAL4 DNA-binding domainExpressionMammalianMutationdeleted amino acids 1-334PromoterpCMVAvailable sinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHsp18.2 (GB0153)
Plasmid#68171PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 promoter as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP8GF-AtTIR1
Plasmid#188427PurposeArabidopsis Nuclear Auxin Signaling Receptor TIR1DepositorInsertAuxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsp70B (GB0155)
Plasmid#68172PurposeProvides the Arabidopsis thaliana heat shock protein 70B promoter as a level 0 GoldenBraid partDepositorInsertAtHsp70B promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJKW1813
Plasmid#174003PurposeExpresses PpCYP90G4 and AtATR1 for reconstitution of diosgenin biosynthesis in yeastDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH131
Plasmid#48260PurposeTemplate plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
GAL10/GAL1 bidirectional promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterGAL10/GAL1 bidirectionalAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTHsp18.2 (GB0035)
Plasmid#68186PurposeProvides the Arabidopsis thaliana heat shock protein 18.2 terminator as a level 0 GoldenBraid partDepositorInsertAtHsp18.2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDGE497
Plasmid#153249PurposeM6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
5mCUC1 pGreenII0129
Plasmid#12067DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UsePlant transformation via agrobacteriumTagsExpressionMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pPAtUbq10 (GB0223)
Plasmid#68174PurposeProvides the Arabidopsis thaliana polyubiquitin 10 promoter as a level 0 GoldenBraid partDepositorInsertAtUbq10 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPAct2noUTR (GB0192)
Plasmid#68177PurposeProvides the Arabidopsis thaliana actin2 gene promoter (without 5' UTR) as a level 0 GoldenBraid partDepositorInsertAtAct2 promoter (no UTR)
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTAtUbq3 (GB0219)
Plasmid#68192PurposeProvides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid partDepositorInsertAtUbq3 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
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5mCUC1 pBluescript
Plasmid#12065DepositorInsertCUC1 mutant (CUC1 Mustard Weed)
UseTagsExpressionBacterialMutationmiR-resistant CUC1. Made by introducing five sile…PromoterAvailable sinceJune 2, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-Ter
Plasmid#211847PurposePlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degronDepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoter35SAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
1BR9-AtWUS
Plasmid#193865PurposeE. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.DepositorInsertAtWUS E. Coli Codon Optimized
UseTags6xHis, GFP11, and R9ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
CDS_Cas9-NLS
Plasmid#136121PurposePuchta Arabidopsis codon-optimised Cas9 for CRISPRDepositorInsertCDS_AtcoCas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAct2 (GB0210)
Plasmid#68191PurposeProvides the Arabidopsis thaliana Actin2 gene terminator as a level 0 GoldenBraid partDepositorInsertAtAct2 terminator
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
p5'UTRAct2 (GB0193)
Plasmid#68178PurposeProvides the 5'-untranslated region of the Arabidopsis thaliana actin2 gene as a level 0 GoldenBraid partDepositorInsert5'UTR AtAct2
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPAt2S3 (GB0029)
Plasmid#68162PurposeProvides the Arabidopsis thaliana Seed-specific promoter 2S3 as a level 0 GoldenBraid partDepositorInsertAt2S3 promoter
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
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MT583
Plasmid#63114PurposeThe gDNA construct is designed for arabidopsis ecotype Est-1 ACD6 expression in other genetic backgrounds.DepositorInsertACD6 (Accelerated Cell Death 6)
UseTagsExpressionPlantMutationPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-AtUBC8-S-AtUBA1-S
Plasmid#222714PurposeExpression AtUBA1-S and AtUBC8-S in in bacterial cellsDepositorUseTagsS-TagExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CRY2(535, L348F)-mCherry
Plasmid#75372PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-535, L348F)-mCherryDepositorInsertCRY2 residues 1-535 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationL348FPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CRY2PHR(W349R)-mCherry
Plasmid#75370PurposeExpresses fusion of Arabidopsis CRY2 (residues 1-498, W349R fast-cycling mutant) fused to mCherryDepositorInsertCRY2 residues 1-498 (CRY2 Mustard Weed)
UseTagsmCherryExpressionMammalianMutationW349RPromoterCMVAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
UseTagsHexa-Histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBG-ttLbUV2
Plasmid#231386PurposeLbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.DepositorInsertttLbCas12a Ultra V2
UseCRISPRTagsExpressionPlantMutationD156R and E795LPromoterAvailable sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
Gal4BD-CRY2(535)
Plasmid#75369PurposeYeast two-hybrid expression vector, expresses Gal4BD fused to Arabidopsis CRY2 (residues 1-535)DepositorInsertCRY2 (CRY2 Mustard Weed)
UseTagsGal4BDExpressionYeastMutationPromoterADH1Available sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry-UBQ
Plasmid#78932PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants. Enable production of two gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
gRNA NIA TLS2
Plasmid#196981PurposeExpresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS2 mobility signal that confers graft mobility.DepositorInsertAtNIA1 gRNAs fused to TLS2 tags
UseTagsExpressionPlantMutationPromoterpU6-26, pU6-29Available sinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only