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Showing: 521 - 540 of 10397 results

  3. pFRT-TODestFLAGHA_RBPMS_KDR_K100A

    Plasmid
    #65663
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation K100A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed lysine 100 to alanine (KDR background)
    Promoter
    CMV, 2x Tet
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    65663-plasmid-map-sequence-id-111631

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  6. pFRT-TODestFLAGHA_RBPMS_KDR_F27A

    Plasmid
    #65661
    Purpose
    FLAG-HA expression vector with RBPMS carrying mutation F27A and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
    Depositor
    Thomas Tuschl
    Article
    Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.
    Insert
    RBPMS (RBPMS Human)
    Use
    Tags
    FLAG HA
    Expression
    Mammalian
    Mutation
    changed phenylalanine 27 to alanine (KDR backgrou…
    Promoter
    CMV, 2x Tet
    Available since
    June 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    65661-plasmid-map-sequence-id-111634

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  8. pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

    Plasmid
    #218513
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of the Plac_mrfp cassette
    Promoter
    PgyrA_ermBL-ermB'
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218513-plasmid-map-sequence-id-444102

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  9. pEC3109 (pSpy1C-Plac(1)_ffluc)

    Plasmid
    #218514
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(1)
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218514-plasmid-map-sequence-id-444100

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  10. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Tags
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  11. pSLC-242

    Plasmid
    #73189
    Purpose
    Template plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.
    Depositor
    Swaine Chen
    Article
    Khetrapal et al Nucleic Acids Res. 2015 Jul 27;43(13):e8
    Insert
    chloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    chloramphenicol promoter
    Available since
    July 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    73189-plasmid-map-sequence-id-153071

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Showing: 521 - 540 of 10397 results