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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,378 results for: pho;

Showing: 521 - 540 of 9378 results

  1. pJK631

    Plasmid
    #72390
    Purpose
    Produces Acetobacter aceti 1023 phosphoribosylpyrophosphate synthetase (AaPrsAH6-insA2), C-terminal His6 tag
    Depositor
    T. J. Kappock
    Insert
    phosphoribosylpyrophosphate synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    inserted Ala2 to facilitate cloning
    Promoter
    T7
    Available Since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    72390-plasmid-map-sequence-id-133987

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  5. pOpen-Endonuclease IV E. coli

    Plasmid
    #165551
    Purpose
    DNA AP endonuclease; Catalyzes the cleavage of DNA phosphodiester backbone at AP sites via hydrolysis leaving a 1 nucleotide gap with 3'-hydroxyl and 5' deoxyribose phosphate (dRP) termini; Also has 3'-diesterase activity which can remove 3' phosphate, 3'-alpha, beta-unsaturated aldehyde, phosphoglycoaldehyde, and other 3' blocking groups.
    Depositor
    FreeGenes Project, Drew Endy, Jennifer Molloy
    Insert
    Endonuclease IV E. coli
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165551-plasmid-map-sequence-id-339868

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  11. pfbiRS

    Plasmid
    #67283
    Purpose
    Expresses the fbiC gene from Mycobacterium smegmatis
    Depositor
    Aziz Sancar
    Article
    Selby et al Biochemistry. 2012 Jan 10;51(1):167-71.
    Insert
    fbiC gene from Mycobacterium smegmatis
    Use
    Tags
    polyhistidine
    Expression
    Mammalian
    Mutation
    non known
    Promoter
    Available Since
    June 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. RCAS IkB alpha M

    Plasmid
    #12407
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Promoter
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  14. (PM-S608D)FLAG-Cep215-FL

    Plasmid
    #106909
    Purpose
    Expresses murine phospho-mimetic CEP215 at S608
    Depositor
    Heidi Hehnly
    Insert
    CEP215 (Cdk5rap2 Mouse)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed Serine-608 to Aspartic Acid
    Promoter
    CMV
    Available Since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    106909-plasmid-map-sequence-id-204099

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  16. PPDK

    Plasmid
    #64037
    Purpose
    Expression of Pyruvate phosphate dikinase (PPDK) in E. coli
    Depositor
    Vern Schramm
    Article
    Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do
    Insert
    pyruvate phosphate dikinase
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    May 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. APRT

    Plasmid
    #64028
    Purpose
    Expression of S. crervisiae strain APRTase in E. coli. Contains N-terminal HistidineX6 tag.
    Depositor
    Vern Schramm
    Article
    Shi et al Biochemistry. 2001 Sep 11;40(36):10800-9
    Insert
    adenine phosphoribosyltransferase (APT1 Budding Yeast)
    Use
    Tags
    Histidine x 6
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pKD‐SepRS-­EFSep-­5x tRNASep (B40 OTS)

    Plasmid
    #52054
    Purpose
    Used in phosphoprotein synthesis. Expresses the Sep-accepting tRNA (tRNASep), the Sep-tRNA synthetase (SepRS) and an engineered EF-Tu (EFSep)
    Depositor
    Jesse Rinehart
    Inserts
    SepRS
    EF-Sep
    5x tRNA Sep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    His67 to Arg , Glu216 to Asn, Asp217 to Gly, Phe2…
    Promoter
    Ptac
    Available Since
    March 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    52054-plasmid-map-sequence-id-438919

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Showing: 521 - 540 of 9378 results