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We narrowed to 1,631 results for: prep

Showing: 521 - 540 of 1631 results

  1. pSL103-SpCas9-FLAG-3XNLS

    Plasmid
    #182032
    Purpose
    Expression of recombinant Streptococcus pyogenes Cas9 protein carrying C-terminal FLAG and 3XNLS for genome editing as Cas9 RNP
    Depositor
    Steven Lin
    Article
    Lin et al Bio Protoc. 2022 May 20;12(10):e4420. do
    Insert
    Streptococcus pyogenes cas9 (cas9 Synthetic)
    Use
    CRISPR
    Tags
    12X His tag, 3XNLS, FLAG, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    182032-plasmid-map-sequence-id-360101

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  3. pMZ7

    Plasmid
    #223180
    Purpose
    pORI28 derivative with lacZ and upp gene inserted into the backbone; for generating gene-specific knockouts.
    Depositor
    Paul Blainey
    Article
    Zhu et al bioRxiv [Preprint]. 2023 Dec 30:2023.12.
    Type
    Empty backbone
    Use
    Chromosomal integration
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    223180-plasmid-map-sequence-id-441239

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  6. pMSCV-BLU-VIPR

    Plasmid
    #220497
    Purpose
    Retroviral plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222-2A-Thy1.1 and the C120 element followed by cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR and Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220497-plasmid-map-sequence-id-442817

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  7. pBLU-VIPR

    Plasmid
    #220498
    Purpose
    Plasmid allowing for potent induction of gRNA using blue light. The plasmid contains VPR-EL222 and the C120 response element followed by mCherry and cloning sites for ribozyme flanked gRNA (RGR).
    Depositor
    Alexander Espinosa
    Article
    Pulgarin et al bioRxiv [Preprint]. 2023 Nov 10:2023.11.
    Type
    Empty backbone
    Use
    CRISPR; Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    220498-plasmid-map-sequence-id-442816

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  10. piggyFlex

    Plasmid
    #218234
    Purpose
    A piggyBac transposon-based gRNA expression vector, to allow for genomic integration and stable expression of gRNAs. Contains both antibiotic (puromycin) and fluorophore (GFP) markers.
    Depositor
    Jay Shendure
    Article
    Chardon et al bioRxiv [Preprint]. 2023 Mar 28:2023.03.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    218234-plasmid-map-sequence-id-432525

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  13. pHPHS232

    Plasmid
    #228356
    Purpose
    Landing pad with Bxb1 attP_wildtype site, Dox-inducible BFP-iCasp9-Blasticidin, CMV-driven rtTA3, T2A-neomycin for HDR into AAVS1 locus
    Depositor
    Arvind Rasi Subramaniam
    Article
    Nugent et al bioRxiv [Preprint]. 2024 Jul 26:2024.07.
    Inserts
    mTagBFP2
    Reverse tetracycline transactivator 3
    neo
    Use
    Tags
    Blasticidin S deaminase and iCasp9
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3GV and gene trap
    Available since
    Jan. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    228356-plasmid-map-sequence-id-455149

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  14. pEM047 [T7-assisted CROPseq sgRNA expression vector]

    Plasmid
    #224899
    Purpose
    Lentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNA
    Depositor
    Thomas Norman
    Article
    Metzner et al bioRxiv [Preprint]. 2024 Jul 27:2024.07.
    Inserts
    eGFP
    LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    224899-plasmid-map-sequence-id-443624

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  20. pSBL_DK186

    Plasmid
    #156422
    Purpose
    Mammalian expression of 3a-EGFP with CMV promoter, can be used for transfection or used to generate bacmids for baculoviral infection of mammalian cells
    Depositor
    Stephen Brohawn
    Article
    Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.
    Insert
    SARS-CoV-2 3a protein
    Use
    Tags
    EGFP-His
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    156422-plasmid-map-sequence-id-306308

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Showing: 521 - 540 of 1631 results