We narrowed to 18,395 results for: Tec

1. pMT492

   Plasmid

   #139397

   Purpose

   Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   Jan. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEC3006 (pSpy1C-PZn_ffluc)

   Plasmid

   #218515

   Purpose

   replicative E. coli-S. pyogenes shuttle plasmid for zinc-inducible expression of the Firefly luciferase in S. pyogenes

   Depositor

   Emmanuelle Charpentier

   Article

   Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

   Insert

   ffluc
   

   Use

   Luciferase

   Tags

   Expression

   Bacterial

   Mutation

   deletion of Plac_mrfp cassette

   Promoter

   PZn (PczcD)

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT493

   Plasmid

   #139398

   Purpose

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMT494

   Plasmid

   #139399

   Purpose

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMT449

   Plasmid

   #139389

   Purpose

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Bile Acid inducible Promoter

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMT455

   Plasmid

   #139392

   Purpose

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR

   Depositor

   Christopher Voigt

   Article

   Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

   Insert

   aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   aTC and BA inducible promoters

   Available since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT9R

   Plasmid

   #98305

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   TRPL3AcNES1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMM0646

   Plasmid

   #129007

   Purpose

   Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker

   Depositor

   Megan McClean

   Article

   An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

   Insert

   KanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pR26 CAG/BFP Dest

   Plasmid

   #74282

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDA_836

    Plasmid

    #216088

    Purpose

    Cas9 [Sp] knockout targeting CD55, positive control

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    CD55 guide
    

    Use

    CRISPR and Lentiviral; Positive controls

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGWB510

    Plasmid

    #74852

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid

    #83485

    Purpose

    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).

    Depositor

    Jennifer Doudna

    Article

    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

    Insert

    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    

    Use

    CRISPR

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    R472A, H477A, R1048A, H1053A

    Promoter

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV1 for Split-PE, SpCas9 nickase (PKC1001)

    Plasmid

    #190111

    Purpose

    AAV1 for expression of nickase SpCas9(H840A) for Split-PE use

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    nSpCas9
    

    Use

    AAV

    Tags

    bpNLS

    Expression

    Mutation

    H840A

    Promoter

    EF1a

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-Foxo1(pCMV5)

    Plasmid

    #12148

    Depositor

    Domenico Accili

    Article

    Kitamura et al Cell Metab. 2005 Sep . 2(3):153-63.

    Insert

    Foxo1 (Foxo1 Mouse)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKD237-3a-2

    Plasmid

    #90957

    Purpose

    Expresses ThsR and mCherry constitutively and sfGFP under the PphsA promoter

    Depositor

    Jeffrey Tabor

    Article

    Daeffler et al Mol Syst Biol. 2017 Apr 3;13(4):923. doi

    Inserts

    Thiosulfate response regulator
    Superfolder GFP
    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Bba_J23100, Bba_J23105, and PphsA

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-HA-GPR35

    Plasmid

    #204708

    Purpose

    expresses HA-GPR35 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    G protein-coupled receptor 35 (GPR35 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGWB413

    Plasmid

    #74807

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 3xHA (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGWB553

    Plasmid

    #74883

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with mRFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    No Promoter

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-Sp-gRNA-AAVS1_B3903c_attP

    Plasmid

    #182144

    Purpose

    To insert attP attachment site at AAVS1 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    AAVS1_B3903c_attP pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pU6-Sp-gRNA-AAVS1_A3786c_attP

    Plasmid

    #182143

    Purpose

    To insert attP attachment site at AAVS1 in human cells via twinPE

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    A3786c-attP pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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