We narrowed to 170,816 results for: htt
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SARS2nsp3_MacroD_ECBio2
Plasmid#234388PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp3_SARS2
TagsLVPRGSSSKGGYGLNDIFEAQKIEWHE and MHHHHHHSSGVDLGTEN…ExpressionBacterialPromoterT7Available SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
CRISPR plasmid
Plasmid#236553PurposeEncodes Cas9 and two guideRNAs, one to cut the genome downstream of HSPA5 (BiP/GRP78) and one to linearize the DONOR plasmid.DepositorInsertCas9
ExpressionMammalianAvailable SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
nsp14_EC_Bio1
Plasmid#234376PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp14_SARS2
TagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionBacterialPromoterT7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS2nsp3_MacroD_EC
Plasmid#234389PurposeBacterial expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp3_SARS2
TagsMHHHHHHSSGVDLGTENLYFQSMExpressionBacterialPromoterT7Available SinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
NL63_NSP13_BV_BIO
Plasmid#234381PurposeBaculovirus expression for structure determination. May not contain entire coding region of gene.DepositorInsertNsp13_NL63
UseBaculovirus expresssionTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGPromoterPolyhedrinAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaX(T-only)-pET(3c)
Plasmid#237237Purposeover-express E. coli PolX (Pol III tau subunit - no gamma)DepositorInsertdnaX (tau only) (dnaX E. coli)
ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec dnaN-pET(11a)
Plasmid#237238Purposeover-express E. coli dnaN (Pol III beta sliding clamp subunit)DepositorInsertdnaN (beta) (dnaN E. coli)
ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Ec holA-pET(11a)(ACH-)
Plasmid#237233Purposeover-express E. coli holA (Pol III delta subunit)DepositorInsertholA (delta) (holA E. coli)
ExpressionBacterialAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSG7084 pG46-46
Plasmid#232621PurposepG46 derivative containing two replacement cassette sites (siteA and siteB) for gap-replacement-based chemical modification(s).DepositorTypeEmpty backboneUsePrecursor plasmid to generate modified dna circlesAvailable SinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 9bp dist
Plasmid#234131PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 12bp dist
Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 27bp dist
Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP001: pLenti CMV Blast::eGFP-RC-I-1-H11
Plasmid#209301PurposeExpression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal eGFPDepositorInsertRC-I-1-H11
UseLentiviralTagseGFPAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EP002: pLenti CMV Blast::HaloTag-RC-I-1-H11
Plasmid#209302PurposeExpression plasmid for RC-I-1-H11 (de novo virus-like particle designed by David Baker lab) with N-terminal HaloTagDepositorInsertRC-I-1-H11
UseLentiviralTagsHaloTagAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
PME-1 S156A (inakt.mut) pcDNA-StrepIII
Plasmid#119293PurposeMammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active centerDepositorAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2643
Plasmid#229527PurposeCarries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mAID_optExpressionBacterial and YeastMutationF74GAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRE-Flex-MCS-WPRE-SV40 - GCH26
Plasmid#230827PurposeAAV cloning vector for CRE-dependent expression under the TRE promoter.DepositorTypeEmpty backboneUseAAV and Cre/Lox; Cloning vectorAvailable SinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
Francisella tularensis enoyl reductase
Plasmid#231104PurposeExpresses FabI under T7 with HIS tagDepositorInsertEnoyl-acyl carrier protein reductase
Tags6x HIS, GSTExpressionBacterialAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE
Plasmid#233047PurposeTo express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' directionDepositorInsertGFP and GluN2B
UseLentiviralTagsFlag and GFPAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLSU2/mIR{PepGMV}:mNeonGreen:NosT
Plasmid#212193PurposeThis binary vector expresses mNeonGreen with a modified IR pepper golden mosaic virus (PepGMV) promoterDepositorInsertTomato codon optimized version of mNeonGreen
ExpressionPlantMutationmNeonGreen codon-optimized for tomatoPromoterPepGMV mIR promoterAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_4J5Q
Plasmid#234233PurposeHeterologous protein expression of ADP-ribose glycohydrolase OARD1 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3OBS
Plasmid#234227PurposeHeterologous protein expression of tumor susceptibility gene 101 protein in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_5GUQ
Plasmid#234171PurposeHeterologous protein expression of ASCH domain-containing ribonuclease in Escherichia coliDepositorInsert5GUQ
Tags10x HisTag-Smt3ExpressionBacterialPromoterT7Available SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_1AKO
Plasmid#234162PurposeHeterologous protein expression of exodeoxyribonuclease III (Exonuclease III) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAIP4_3V46
Plasmid#234165PurposeHeterologous protein expression of cell division control protein 73 in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
mP_ILB_Vector 1
Plasmid#232193PurposeThe plasmid vector designed for the expression of four genes in oleaginous yeasts, including Yarrowia lipolytica and Rhodotorula toruloides.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastAvailable SinceMarch 3, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGL3_SFRP1
Plasmid#232357PurposeEnhancer near SFRP1, chr8:41338016-41339065 cloned into pGL3DepositorInsertSFRP1 (SFRP1 Human)
UseLuciferaseAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_RNF157_SOX9scr_neg0str
Plasmid#232373PurposeRNF157 enhancer, SOX9 site scrambled, buffering site strengthenedDepositorInsertRNF157 (RNF157 Human)
UseLuciferaseMutationRNF157 enhancer, SOX9 site scrambled, buffering s…Available SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_THADA_allscramble
Plasmid#232386PurposeTHADA enhancer, all sensitizing elements scrambledDepositorInsertTHADA (THADA Human)
UseLuciferaseMutationTHADA enhancer, all sensitizing elements scrambledAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3_RNF157_neg0SOX9scramble
Plasmid#232379PurposeRNF157 enhancer, SOX9 site and buffering site scrambledDepositorInsertRNF157 (RNF157 Human)
UseLuciferaseMutationRNF157 enhancer, SOX9 site and buffering site scr…Available SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
FtHAP-pNIC-Bsa4
Plasmid#86778PurposeExpresses Francisella tularensis Histidine Acid Phosphatase under T7 with HIS tagDepositorInsertHistidine Acid Phosphatase
Tags6x His tagExpressionBacterialAvailable SinceFeb. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
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1203T-CMV-Cas7.1-NLS-bGHpolyA
Plasmid#221540PurposeEffector only expresses Cas7.1 domain (truncated from DiCas7-11 effector), nuclear localization signal sequence on 5' and 3' end of the coding domainDepositorInsertTruncated DiCas7-11
UseCRISPRExpressionMammalianAvailable SinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only
