We narrowed to 9,135 results for: Pol

1. pOPINS-USP21 (USP, aa 196-565)

   Plasmid

   #61585

   Purpose

   Expresses human USP21 (USP domain) in E. coli.

   Depositor

   David Komander

   Article

   Ye et al EMBO Rep. 2011 Apr;12(4):350-7. doi: 10.

   Insert

   USP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
   

   Tags

   His6-SUMO

   Expression

   Bacterial

   Mutation

   Deleted aa 1-195.

   Promoter

   T7

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV BARK D110A p2A mCherry

   Plasmid

   #117690

   Purpose

   CMV-iBARK(D110A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

   Depositor

   Baljit Khakh

   Article

   Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

   Insert

   BARKrgs D110A peptide
   

   Tags

   HA / FLAG

   Expression

   Mammalian

   Mutation

   D110A

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTSlb-wt

   Plasmid

   #60728

   Purpose

   Expresses both fragments of T7 RNAP split at position 179. The N-terminal fragment is driven by PLac while the C-terminal fragment is driven by PBAD.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Inserts

   Residues 1-179 of split T7 RNAP
   Residues 180-880 of split T7 RNAP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   Residues 1-180 of split T7 RNAP and Residues 180-…

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 human cofilin S3E

   Plasmid

   #50855

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available Since

   Feb. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

5. W118-1_hTGM2-V5

   Plasmid

   #180413

   Purpose

   lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal V5 tag) gene

   Depositor

   Huda Zoghbi

   Article

   Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

   Insert

   TGM2 (TGM2 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1A-C1A-EYFP

   Plasmid

   #61155

   Purpose

   Diacylglycerol (DAG) reporter

   Depositor

   Tobias Meyer

   Article

   Tsai et al Nat Cell Biol. 2014 Feb;16(2):133-44. do

   Insert

   C1A-C1A-EYFP, C1A from PKCG (aa 26-89, rat) (Prkcg Rat)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   C1A from PKCG amino acids 26-89

   Promoter

   CMV

   Available Since

   Dec. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR660

   Plasmid

   #65216

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-8 cap
   

   Use

   AAV

   Expression

   Insect

   Promoter

   P10 and polyhedrin

   Available Since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P-2-human RNF146 full length

   Plasmid

   #132610

   Purpose

   expresses human RNF146 full length in E. coli

   Depositor

   Wenqing Xu

   Article

   DaRosa et al Nature. 2015 Jan 8;517(7533):223-6. doi:

   Insert

   RNF146 (RNF146 Human)
   

   Expression

   Bacterial

   Available Since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-20b-M-Ntx-S1

   Plasmid

   #58716

   Purpose

   Expresses in Escherichia coli Notexin with a N-terminal extra methionine and with the amino acid substitution N1S

   Depositor

   Fiorella Tonello

   Article

   Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

   Insert

   Notexin
   

   Tags

   Methionine

   Expression

   Bacterial

   Mutation

   Changed Asparagine 1 to Serine

   Promoter

   T7

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P-1-eIF4E K119A

    Plasmid

    #199440

    Purpose

    Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

    Depositor

    Shin-ichi Hoshino

    Article

    Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

    Insert

    1977 (EIF4E Human)
    

    Tags

    GST-fusion protein

    Expression

    Bacterial

    Mutation

    changed lysine 119 to alanine

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. RDX-mCherry

    Plasmid

    #214476

    Purpose

    Plasmid for transient transfection of RDX-mCherry fusion protein in mammalian cells.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    RDX (RDX Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Sequence matches cDNA clone MGC:48283 IMAGE:52844…

    Promoter

    CMV

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV-mir-17-92

    Plasmid

    #64100

    Purpose

    expresses mir-17-92

    Depositor

    Lin He

    Article

    Olive et al Elife. 2013 Oct 15;2:e00822. doi: 10.755

    Insert

    miR-17-92 (MIR17HG Human)
    

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-Hyg-Dest-mCherry-PAR-6B

    Plasmid

    #194908

    Purpose

    To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

    Depositor

    Clare Buckley

    Article

    Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

    Insert

    PAR-6B (Pard6b Mouse)
    

    Use

    Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    39 C to G, silent mutation

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQHA-USP11 WT puroR

    Plasmid

    #46749

    Purpose

    Retroviral vector that expresses wild type HA-tagged wild type human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-DIO-TVA-V5-RG

    Plasmid

    #119743

    Purpose

    Expresses TVA, V5, and RG in a cre dependent manner.

    Depositor

    Marie Carlén

    Article

    Ahrlund-Richter et al Nat Neurosci. 2019 Apr;22(4):657-668. do

    Insert

    TVA, V5, Rabies Glycoprotein
    

    Use

    AAV and Cre/Lox

    Tags

    V5

    Available Since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-SYN-H-sDarken

    Plasmid

    #184797

    Purpose

    High affinity version of the Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    H-sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV6-AN-HA_Ubiquitin

    Plasmid

    #131258

    Purpose

    Mammalian expression of ubiquitin with an N-terminal HA tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin C (UBC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. TetO-FUW-PBRM1-pgk-puro

    Plasmid

    #85746

    Purpose

    tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

    Depositor

    Emily Dykhuizen

    Article

    Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

    Insert

    PBRM1 (PBRM1 Human)
    

    Use

    Lentiviral

    Tags

    none

    Promoter

    CMV

    Available Since

    March 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available Since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSR651

    Plasmid

    #65213

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-1 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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