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pDONR223_CCNG1_p.E167K

Plasmid

#81326

Purpose

Gateway Donor vector containing CCNG1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CCNG1 (CCNG1 Human)

Use

Gateway Cloning

Mutation

E167K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDK4_p.A23S

Plasmid

#81327

Purpose

Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDK4 (CDK4 Human)

Use

Gateway Cloning

Mutation

A23S

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_CDKN1B_p.C29F

Plasmid

#81307

Purpose

Gateway Donor vector containing CDKN1B , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

CDKN1B (CDKN1B Human)

Use

Gateway Cloning

Mutation

C29F, V109G

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ING1_WT

Plasmid

#82213

Purpose

Gateway Donor vector containing ING1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ING1 (ING1 Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RAF1_p.L613V

Plasmid

#82180

Purpose

Gateway Donor vector containing RAF1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RAF1 (RAF1 Human)

Use

Gateway Cloning

Mutation

L613V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHOA_p.T19N

Plasmid

#82182

Purpose

Gateway Donor vector containing RHOA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHOA (RHOA Human)

Use

Gateway Cloning

Mutation

T19N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_ARAF_WT

Plasmid

#82096

Purpose

Gateway Donor vector containing ARAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ARAF (ARAF Human)

Use

Gateway Cloning

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3R3_WT

Plasmid

#82044

Purpose

Gateway Donor vector containing PIK3R3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3R3 (PIK3R3 Human)

Use

Gateway Cloning

Mutation

D338A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_NBPF15_WT

Plasmid

#82068

Purpose

Gateway Donor vector containing NBPF15 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

NBPF15 (NBPF15 Human)

Use

Gateway Cloning

Mutation

D477A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_HRAS_p.G12A

Plasmid

#81277

Purpose

Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

HRAS (HRAS Human)

Use

Gateway Cloning

Mutation

G12A

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_B2M_p.A6V

Plasmid

#81263

Purpose

Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Publication

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

B2M (B2M Human)

Use

Gateway Cloning

Mutation

A6V

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Publication

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Ai161 (TIT2L-GFP-ICR-tTA2) targeting vector

Plasmid

#114429

Purpose

Target a Cre-dependent GFP cassette and Dre-dependent tTA2 cassette to the mouse TIGRE locus

Depositor

Hongkui Zeng

Publication

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Use

Cre/Lox and Mouse Targeting

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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AiP1046-pAAV-mscRE13-minBGpromoter-iCre-WPRE-hGHpA

Plasmid

#163478

Purpose

Direct-expressing iCre AAV Virus. Alias: AiP1046 - pAAV-AiE2013m-minBG-iCre-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

iCre

Use

AAV

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 4x

Plasmid

#20930

Depositor

Yukihide Tomari

Publication

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

4x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pV1393

Plasmid

#111430

Purpose

CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

Depositor

Gerald Fink

Publication

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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AiP1050-pAAV-mscRE16-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163483

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1050 - pAAV-AiE2016m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP1048-pAAV-mscRE10-minBGpromoter-tTA2-WPRE-hGHpA

Plasmid

#163481

Purpose

Direct-expressing tTA2 AAV Virus. Alias: AiP1048 - pAAV-AiE2010m-minBG-tTA2-WPRE-HGHpA

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Publication

Graybuck et al Neuron. 2021 Mar 23. pii: S0896-6273(21)

Insert

tTA2

Use

AAV

Promoter

Beta Globin minimal promoter

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_nHA Spinophilin

Plasmid

#87122

Purpose

mammalian expression of Spinophilin

Depositor

Anthony Baucum

Publication

Hiday et al Neural Plast. 2017;2017:4153076. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Spinophilin (PPP1R9B Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pUC-GFP-HS

Plasmid

#133305

Purpose

"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

Depositor

Chris Barnes

Publication

Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

Insert

hok/sok toxin-antitoxin cassette

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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