We narrowed to 9,135 results for: Pol

1. pNSSCPG

   Plasmid

   #183187

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 p

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   superfolder GFP
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA pDEST40 Cas9-HMGB1

   Plasmid

   #183195

   Purpose

   Expression clone

   Depositor

   Emma Haapaniemi

   Article

   Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

   Insert

   HMGB1
   

   Use

   CRISPR

   Tags

   Cas9WT

   Expression

   Mammalian

   Mutation

   n/a

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. p3xFlag-Alkbh4

   Plasmid

   #105240

   Purpose

   Expresses murine Alkbh4 containing an N-ternimal 3xFlag tag

   Depositor

   Douglas Feldman

   Article

   Kweon et al Mol Cell. 2019 Apr 5. pii: S1097-2765(19

   Insert

   Alkbh4 (Alkbh4 Mouse)
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAP03-act1p

   Plasmid

   #120233

   Purpose

   Carries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.

   Depositor

   Bradley Moore

   Article

   Bauman et al Cell Chem Biol. 2019 Feb 15. pii: S2451-

   Insert

   promoter cassette containing act1p promoter and apramycin resistance gene
   

   Use

   Synthetic Biology

   Mutation

   aac(3)IV (apramycin resistance gene)

   Available Since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+)_UGControl

   Plasmid

   #133875

   Purpose

   Expresses UGControl in mammalian cells

   Depositor

   Sumio Terada

   Article

   Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

   Insert

   UtrCH (UTRN Human)
   

   Tags

   flexible linker - mEGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 TFAM

   Plasmid

   #67064

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TFAM (TFAM Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Mutation

   S115C

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA1 Flag ATXN1L

   Plasmid

   #33242

   Depositor

   Huda Zoghbi

   Article

   Bowman et al Nat Genet. 2007 Mar;39(3):373-9. Epub 20

   Insert

   ATXN1L (ATXN1L Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   April 17, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pBa.GFP-AP1/beta1

   Plasmid

   #218802

   Purpose

   Mammalian expression o fhuman AP1 b1

   Depositor

   Marvin Bentley

   Article

   Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

   Insert

   AP1 b1 (AP1B1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   Chicken Beta actin

   Available Since

   May 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-CAG-DD-3xFLAG-hSOX17-GS-TagBFP-BGHpA

   Plasmid

   #172226

   Purpose

   Expression vector with Shield-1 stabilizable hSOX17 fused to TagBFP.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Insert

   SOX17 (SOX17 Human)
   

   Use

   Piggybac transposon

   Tags

   3XFLAG, DD-domain, GS-linker, and TagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDule-IBBN (G2)

    Plasmid

    #85500

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. A3B-Cas9n-UGI-NLS

    Plasmid

    #198889

    Purpose

    Express full-length A3B BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
    

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. -227 hTF (mutant NF-kB)

    Plasmid

    #15443

    Depositor

    Nigel Mackman

    Article

    Oeth et al Arterioscler Thromb Vasc Biol. 1997 Feb

    Insert

    Tissue factor promoter (F3 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    NF-kappaB binding domain mutated

    Available Since

    July 27, 2007

    Availability

    Academic Institutions and Nonprofits only

13. Lenti-SLC35B2-sgRNA

    Plasmid

    #154860

    Purpose

    Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 sgRNA (SLC35B2 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. psicheck-2-Casp8

    Plasmid

    #48172

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    5" UTR of CASP8 (CASP8 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

15. sg1+sg2+sg3

    Plasmid

    #113969

    Purpose

    Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    sg1+sg2+sg3
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV Puro SCRIB P305L

    Plasmid

    #88887

    Purpose

    SCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backbone

    Depositor

    Senthil Muthuswamy

    Article

    Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

    Insert

    SCRIB (SCRIB Human)
    

    Use

    Retroviral

    Mutation

    Proline to Leucine mutation @ 305th amino acid

    Promoter

    MSCV promoter

    Available Since

    March 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hsp70:Stathmin1-mKate2

    Plasmid

    #105969

    Purpose

    heat shock inducible transgenesis, stathmin stmn1 orig. from addgene #31326

    Depositor

    Caren Norden

    Article

    Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

    Insert

    Stathmin1 (STMN1 Human)
    

    Tags

    mKate2

    Expression

    Bacterial

    Available Since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO-mCherry MALAT1_Enhancer.1

    Plasmid

    #78536

    Purpose

    Experimental plasmid

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    gRNA1+scaffold+H1promoter+gRNA2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6 (for expressing sgRNA) and U6 promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. EGFP-Hu_Plk5(WT)

    Plasmid

    #136319

    Purpose

    Mammalian expression of human Plk5-WT with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Article

    de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

    Insert

    PLK5 (PLK5 Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-Enhancer-ZC3H12D_PromoterWT

    Plasmid

    #153066

    Purpose

    ZC3H12D promoter region cloned upstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    ZC3H12D promoter region (ZC3H12D Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    none

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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