We narrowed to 13,645 results for: NTS

1. lenti-Cas9-VQR-Blast

   Plasmid

   #87155

   Purpose

   lentiviral expression of SpCas9-VQR (NGA PAM restricted)

   Depositor

   Daniel Bauer

   Article

   Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

   Insert

   SpCas9-VQR(D1135V,R1335Q,T1337R)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D1135V,R1335Q,T1337R

   Promoter

   Available since

   Feb. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4938

   Plasmid

   #107527

   Purpose

   Expresses Fn crRNA and mCherry in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CBh and human U6

   Available since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV_EF1a_CDK1-P2A-Hygro_Barcode

   Plasmid

   #170221

   Purpose

   Barcoded lentiviral vector to express CDK1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   CDK1 (CDK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 416GPD-FUS-YFP

   Plasmid

   #29594

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 18, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pTE4495

   Plasmid

   #80338

   Purpose

   mammalian expression MbCpf1 nuclease and Mb crRNA

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Mb crRNA
   MbCpf1
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   Aug. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3019_pETcon-SARS-CoV-2-RBD_E484K

   Plasmid

   #184404

   Purpose

   yeast surface display of the SARS-CoV-2 Eta variant RBD

   Depositor

   Jesse Bloom

   Article

   Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

   Insert

   SARS-CoV-2 Eta Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   E484K

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)

   Plasmid

   #160622

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLL

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s_MS2:EDLL_Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTIGER_tdTomato::3xFLAG::dCrk

   Plasmid

   #131138

   Purpose

   UASp construct for over-expression of Drosophila Crk with N-terminal tandem Tomato and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

   Depositor

   Mark Peifer

   Article

   Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

   Insert

   Crk (Crk Fly)
   

   Use

   Tags

   3XFLAG and tdTomato

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 LIC 6A FLAG-TEV-DPP9S LL100EE

   Plasmid

   #166798

   Purpose

   Mammalian cell expression vector for FLAG-tagged DPP9S. LL100EE mutant is loss of CARD8 and NLRP1 binding.

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Use

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   LL100EE

   Promoter

   CMV

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cav2.2 D122A HA pCAGGS

    Plasmid

    #206097

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV/B-actin

    Available since

    Nov. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Cav2.2 HA D122A pcDNA3

    Plasmid

    #206108

    Purpose

    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

    Depositor

    Annette Dolphin

    Article

    Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D122A

    Promoter

    CMV

    Available since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS-mTagRFP-TUBA1B

    Plasmid

    #62850

    Purpose

    Donor vector for gene editing at human TUBA1B locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    mTagRFP-TUBA1B (TUBA1B Synthetic, Human)
    

    Use

    Yeast-e.coli shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.GFAP.SF-iGluSnFR.S72A

    Plasmid

    #106194

    Purpose

    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Insert

    SF-iGluSnFR.S72A
    

    Use

    AAV

    Tags

    Expression

    Mutation

    GltI: S72A

    Promoter

    GFAP

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. FusionRed-Caveolin-C-10

    Plasmid

    #56103

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid

    #191755

    Purpose

    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)

    Depositor

    Irene Tiemann-Boege

    Article

    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

    Insert

    mGFP-FGFR3IIIc
    

    Use

    Tags

    mGFP

    Expression

    Mammalian

    Mutation

    c.1138G>A (G380R) + c.1131C>T (silent mutat…

    Promoter

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FusionRed-CD36-C-10

    Plasmid

    #56104

    Purpose

    Localization: Membrane, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    CD36 (CD36 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pFRT-DHX36iso2E335A

    Plasmid

    #159588

    Purpose

    Express N-ter FLAG HA tagged DHX36 isoform 2 with E335A mutation in mammalian cells

    Depositor

    Markus Hafner

    Article

    Sauer et al Nat Commun. 2019 Jun 3;10(1):2421. doi:

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    E335A

    Promoter

    CMV

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)_Paragranulin+linker 1

    Plasmid

    #176915

    Purpose

    express Paragranulin with linker 1 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Paragranulin + linker 1 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1(+)_Paragranulin-no linker

    Plasmid

    #176917

    Purpose

    express Paragranulin without linker 1 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Paragranulin (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. psiCHECK2-E2F1 3'UTR

    Plasmid

    #29468

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F1 3'UTR (E2F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

21. psiCHECK2-p27Kip1 3'UTR

    Plasmid

    #29476

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    p27 Kip1 3'UTR (CDKN1B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pX131

    Plasmid

    #167149

    Purpose

    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    4'-phosphopantetheinyl transferase, npgA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTwist-SARS-CoV-2 Δ18 B.1.429

    Plasmid

    #169461

    Purpose

    Encodes SARS-CoV-2 B.1.429 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.429 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTwist-SARS-CoV-2 Δ18 B.1.1.298

    Plasmid

    #169460

    Purpose

    Encodes SARS-CoV-2 B.1.1.298 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Garcia-Beltran et al Cell. 2021 Mar 12. pii: S0092-8674(21)00

    Insert

    SARS-CoV-2 B.1.1.298 Spike truncated to remove 18 amino acids (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pmKikGR Actin

    Plasmid

    #105315

    Purpose

    Photoconvertable cytoskeletal protein actin to study kinetics or dynamics

    Depositor

    Kenneth Yamada

    Article

    Doyle et al J Cell Sci. 2012 May 1;125(Pt 9):2244-56

    Insert

    actin beta (ACTB Human)
    

    Use

    Tags

    KikGR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDisplay-EGFP-2A-HA-Nrxn3-alpha- 2(-)/3(+)/4(+)/6(-)-GPI

    Plasmid

    #184335

    Purpose

    Expression of GPI-anchored form of mouse Nrx3-alpha containing the alternative splice site 5 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,3,

    Depositor

    Peter Scheiffele

    Article

    Hauser et al Neuron. 2022 May 8. pii: S0896-6273(22)0

    Insert

    Nrxn3-alpha-GPI (Nrxn3 Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FB009

    Plasmid

    #119707

    Purpose

    Transcriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    PtrpC::nptII::Ttub
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mCherry-hPMCA2xb

    Plasmid

    #47751

    Purpose

    mammalian expression of mCherry tagged hPMCA2, xb splice variant

    Depositor

    Emanuel Strehler

    Article

    Paszty et al Sci Signal. 2015 Feb 17;8(364):ra19. doi

    Insert

    PMCA2x/b (ATP2B2 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    xb splice variant

    Promoter

    CMV

    Available since

    Nov. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

29. pTE4999

    Plasmid

    #107555

    Purpose

    Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hFnCpf1(dead1)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    D917A

    Promoter

    CMV

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. GST-TEV-FUS-1-373aa

    Plasmid

    #29631

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-373aa (FUS Human)
    

    Use

    Tags

    GST and TEV

    Expression

    Bacterial

    Mutation

    Contains FUS aa# 1-373

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only