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pET28-His6-SUMO-Hck-SH2-AviTag

Plasmid

#214206

Purpose

Bacterial expression of SH2 domain of the Hck kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

p60-HCK (HCK Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1089

Plasmid

#242497

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 226

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1022

Plasmid

#242503

Purpose

Expression of ZZ-3xTEV-HsATG14 and ZZ-3xTEV-HsBECN1 in mammalian cells

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Inserts

ATG14 (ATG14 Human)
BECN1 (BECN1 Human)

Tags

ZZ-3xTEV

Expression

Mammalian

Promoter

CAG and CMV

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP768

Plasmid

#242496

Purpose

Bacterial expression of GST-ScAtg38 (87-226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 87-226 only

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO780

Plasmid

#242481

Purpose

Expression of ScATG38 (FL)-2xEGFP under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

2xEGFP

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1091

Plasmid

#242499

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 181)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 181

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO785

Plasmid

#242488

Purpose

Expression of ScAtg38 (FL)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1093

Plasmid

#242501

Purpose

Bacterial expression of GST-ScAtg38 Δ(123 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 123 to 209

Available Since

Sept. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pOP775

Plasmid

#242495

Purpose

Bacterial expression of GST-ScAtg38 (1-78)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

aa 1-78 only

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1092

Plasmid

#242500

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 209)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 209

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1090

Plasmid

#242498

Purpose

Bacterial expression of GST-ScAtg38 Δ(182 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 182 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1094

Plasmid

#242502

Purpose

Bacterial expression of GST-ScAtg38 Δ(210 to 226)

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

GST

Expression

Bacterial

Mutation

deletion of aa 210 to 226

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGS-BacA-23012

Plasmid

#109180

Purpose

Plasmid for expression of proteins under the control of a minimal HSP70 promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Expression

Insect

Promoter

minimal HSP70 promoter

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP796

Plasmid

#235701

Purpose

Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 MIT domain (NRBF2 Human)

Tags

His6(TEV)

Expression

Bacterial

Mutation

aa 1-89

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pYO1645

Plasmid

#235719

Purpose

Protein expression of GST-tagged PX domain (Cys at 2 and C85S) in bacteria

Depositor

Roger Williams

Article

Buckles et al Biophys J. 2020 Dec 1;119(11):2205-2218.

Insert

p40phox PX domain (NCF4 Human)

Tags

GST

Expression

Bacterial

Mutation

Cys at 2, C85S, aa 2-149

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pPBpuro-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209919

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbleo-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209920

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-miRFP703-eDHFR(69K6)-cRaf

Plasmid

#209921

Purpose

Mammalian expression of cRaf fused to miRFP703-eDHFR(69K6)

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

miRFP703-eDHFR(69K6)-cRaf (RAF1 Human)

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTrcHis_VectorA_short_speGpromoter_PA4114

Plasmid

#220474

Purpose

heterologous expression of PA4114 under endogenous speG E. coli promoter

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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