We narrowed to 8,311 results for: Dos
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Plasmid#170341PurposeGFP(UST)_EPACdDEPCD_mCherry biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertGFP(UST)_EPACdDEPCD_mCherry (RAPGEF3 Human)
ExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable SinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)
Plasmid#133792PurposeU6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015DepositorInsertSt3Cas9 sgRNA entry vector
ExpressionMammalianMutationsgRNA architecture from Muller et al. Molecular T…PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCPP6533
Plasmid#128734PurposeGateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal Cya tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3037
Plasmid#122544PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3043
Plasmid#122546PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT3102
Plasmid#122548PurposeExpression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT2999
Plasmid#122535PurposeExpression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2DepositorAvailable SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RVR
Plasmid#108863PurposeFnCpf1 Gateway entry plasmid with N623R, K629R and N633R mutationsDepositorInsertFnCpf1-RVR (FnCpf1 with N623R, K629R and N633R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R, K629R and N633R, Cpf1 is rice codon optimi…Available SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsHis-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGA2ox2:GUS
Plasmid#108564PurposeArabidopsis transformation, expresses GA2ox2 promoter/GUS fusion to study expression pattern in rootsDepositorAvailable SinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGS_N_3xFLAG
Plasmid#101654PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cellsDepositorInsertLRP6 Signal peptide, KpnI linker, 3xFLAG tag
Tags3xFLAG tagExpressionInsectAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRT1-Mb2YFP-2A (IG153)
Plasmid#99619PurposeTo clone gene of interest downstream of FRT1-Mb2YFP-2A cassetteDepositorInsertMb2EYFP
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
FRT2-Mb2Tomato-2A (IG154)
Plasmid#99620PurposeTo clone gene of interest downstream of FRT2-Mb2Tomato-2A cassetteDepositorInsertMb2Tomato
TagsT2AExpressionMammalianAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM4731
Plasmid#85127PurposeNSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.DepositorInsertPkaiBC-KaiB
ExpressionBacterialAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmMextli
Plasmid#79262PurposeExpresses EGFP-tagged DmMextli in S2 cellsDepositorInsertDmMextli (mxt Fly, NM_164598)
UseSchneider 2 cell expressionTagsEGFPPromoterAc5 promoterAvailable SinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
RalA (G23V)( full )-pcw107-V5
Plasmid#64645Purpose(control) when used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pClneoMyc MOB1 T35A
Plasmid#47937Purposeexpresses Myc tagged human MOB1 containing T35A mutationDepositorAvailable SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
(P4)TT
Plasmid#253857PurposerrnB T1 terminator derived from pTrc99ADepositorInsertrrnB T1 terminator derived from pTrc99A
ExpressionBacterialAvailable SinceJune 5, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLJSRSF7-T7-6xHis-SUMO-MRT-CBD
Plasmid#253350PurposeFor the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.DepositorInsert6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.
Tags6xHis-SUMO and CBDExpressionBacterialPromoterT7Available SinceMay 11, 2026AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hNC-gRNA2
Plasmid#249201PurposesgRNA controlDepositorInsertNon-Targeting
UseCRISPR and LentiviralPromoterU6Available SinceApril 15, 2026AvailabilityAcademic Institutions and Nonprofits only