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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,801 results for: pET

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Showing: 5621 - 5640 of 9801 results

  1. MAP1LC3A

    Plasmid
    #25185
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh67.001.121 (MAP1LC3A Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 9, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. pCali2

    Plasmid
    #86119
    Purpose
    Multiplex temperature-gated expression of mWasabi and mCherry reporters
    Depositor
    Mikhail Shapiro
    Article
    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi
    Inserts
    TlpA36
    mWasabi
    TcI
    mCherry
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    A67T and D341A, P60L, D135V, K187R, K202I, L208Q
    Promoter
    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    86119-plasmid-map-sequence-id-173197

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  5. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  8. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Gerhard Wagner
    Insert
    EIF4A1 (EIF4A1 Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133440-plasmid-map-sequence-id-267708

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  9. UHRF1

    Plasmid
    #28163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    UHRF1 (UHRF1 Human)
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    tandem tudor domain
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pSV40_HA-mPKR

    Plasmid
    #120538
    Purpose
    protein expression
    Depositor
    Petr Svoboda
    Insert
    PKR (Eif2ak2 Mouse)
    Expression
    Mammalian
    Available Since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120538-plasmid-map-sequence-id-233982

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  11. CBX6

    Plasmid
    #25296
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX6_02 (CBX6 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 16, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pMSGV-mCD105scFv-myc-CD28HTMcostim-T2A-Thy1.1

    Plasmid
    #193942
    Purpose
    a fully murine CAR targeting CD105 (endoglin), a component of the TGFβ receptor expressed on the surface of certain solid tumors and acute leukemias.
    Depositor
    Greg Delgoffe
    Article
    Lontos et al Oncoimmunology. 2022 Oct 18;11(1):213122
    Insert
    heavy- and light-chain regions of the MJ7/18 hybridoma and constructed a single chain variable fragment (scFv)
    Use
    Retroviral
    Available Since
    May 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    193942-plasmid-map-sequence-id-506308

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  15. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  16. 4R3H

    Plasmid
    #64652
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    YTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 29, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 5621 - 5640 of 9801 results