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rs699-major

Plasmid

#113357

Purpose

evaluate FOXA1 DIV motif enhancer activity in SNP rs6990531 major allele (T)

Depositor

Ralf Jauch

Publication

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

rs6990531 major allele

Use

Luciferase

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha-HA-TFAP4(E135A/S139A)

Plasmid

#110159

Purpose

Lentiviral vector for expression of human TFAP4 (E135A/S139A) in mammalian cells

Depositor

Daniele Guardavaccaro

Publication

D'Annibale et al J Biol Chem. 2014 Mar 14;289(11):7730-7.

Insert

TFAP4 (transcription factor AP-4) (TFAP4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

E135A/S139A

Promoter

CMV

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Publication

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-INCENP d543-746

Plasmid

#108473

Purpose

expression of vsv-INCENP d543-746

Depositor

Susanne Lens

Publication

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

INCENP d543-746 AA (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

Delta Alpha helix, aa543-746 deleted

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Publication

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-AuroraB L154A/H250Y

Plasmid

#108489

Purpose

expression of vsv-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Publication

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pMCh-695

Plasmid

#92263

Purpose

Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

dGW182 PAM2 mut (gw Fly)

Tags

NHA

Expression

Insect

Mutation

The following point mutations were introduced: EF…

Promoter

Ac5

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-607

Plasmid

#92258

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED 7W7A with seven mutated W-motifs in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-633

Plasmid

#92253

Purpose

Plasmid for expression of GST-tagged human TNRC6C 7W7A CED with seven mutated W-motifs in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-664

Plasmid

#92252

Purpose

Plasmid for expression of GST-tagged human TNRC6C CED with mutated PAM2 motif in mammalian cells

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

GST

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

EF1A

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMCh-658

Plasmid

#92257

Purpose

Plasmid for expression of NHA-tagged human TNRC6C CED in mammalian cells with mutated PAM2 motif

Depositor

Marina Chekulaeva, Witold Filipowicz

Publication

Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

Insert

TNRC6C CED (TNRC6C Human)

Tags

NHA

Expression

Mammalian

Mutation

C-terminal effector domain (CED): aa 1369-1690 wi…

Promoter

CMV

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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hSTARR-seq_SCP1 vector_blocking 2

Plasmid

#99317

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Publication

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (antisense)

Plasmid

#83817

Purpose

contains antisense mouse GATA3

Depositor

Douglas Engel

Publication

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3-antisense strand (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pPD95.75 RSF-1

Plasmid

#66863

Purpose

Generate transgenic C..elegans expressing GFP-fused RSF-1

Depositor

Yutaka Hata

Publication

Iwasa et al Exp Cell Res. 2013 Feb 1;319(3):1-11. do

Insert

RSF-1 (rsf-1 Nematode)

Tags

GFP

Expression

Worm

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHA_RBPMS_KDR_111del

Plasmid

#65659

Purpose

FLAG-HA expression vector with RBPMS carrying deletion at amino acid 111 (1-111) and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)

Depositor

Thomas Tuschl

Publication

Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Insert

RBPMS (RBPMS Human)

Tags

FLAG HA

Expression

Mammalian

Mutation

introduced stop codon such as to produce deletion…

Promoter

CMV, 2x Tet

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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Gal4pBS13-Pleum

Plasmid

#60332

Purpose

Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter.

Depositor

Hal Alper

Publication

Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

Insert

promoter

Use

Synthetic Biology; Yeast synthetic hybrid promote…

Expression

Yeast

Available since

May 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCVD403

Plasmid

#63740

Purpose

Contains the LT gene probe (HincII fragment) cloned on BamHI linkers into pACYC184

Depositor

James Kaper

Publication

Gicquelais et al J Clin Microbiol. 1990 Nov;28(11):2485-9

Insert

LT gene probe

Expression

Bacterial

Available since

April 6, 2015

Availability

Academic Institutions and Nonprofits only
pSBbi-FoxO1_1R_13A_3D

Plasmid

#106279

Purpose

Fluorescent fusion protein for FoxO1

Depositor

Laura Heiser

Publication

Gross et al Cell Syst. 2019 Dec 18;9(6):580-588.e4.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Foxo1a (Foxo1 Mouse)
NLS-mCherry-NLS-P2A-Puromycin

Tags

Clover fluorescent protein and NLS

Expression

Mammalian

Mutation

Deleted amino acids 401-636, T24A, S209A, H212R, …

Promoter

EF1a/RPBSA

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2

Plasmid

#66948

Purpose

Mammalian expression of rat ErbB2 tagged with YFP

Depositor

Mads Lerdrup, Bo van_Deurs

Publication

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ErbB2 (Erbb2 Rat)

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

(See depositor comment below on mutations A24T an…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
MG-miR125b-sponge-bulge

Plasmid

#45790

Depositor

David Baltimore

Publication

Chaudhuri et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

miR125b sponge

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles