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pcDNA-HSF1Δ∼PRD

Plasmid

#71725

Purpose

plasmid expressing mic-his tag phosphorylation-deficient hHSF1

Depositor

Lea Sistonen

Publication

Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

Insert

phosphorylation-deficient HSF1 (HSF1 Human)

Tags

mac 6xHIS

Expression

Mammalian

Mutation

15 phosphorylation sites in RD mutated to Alanine

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

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Blue opsin mcherry ---- pcDNA3.1

Plasmid

#62932

Purpose

Blue opsin tagged with mcherry on the C terminus

Depositor

Narasimhan Gautam

Publication

Karunarathne et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

blue opsin

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pWZL Sox2 R74P

Plasmid

#26355

Depositor

Matthew Meyerson

Publication

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R74P

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV4Neo-murine IL-17RC(FL)

Plasmid

#46864

Purpose

expresses mouse IL-17RC (all exons)

Depositor

Sarah Gaffen

Publication

Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL-17RC (Il17rc Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RV-Cag-Dio-mCherry

Plasmid

#87664

Purpose

retroviral vector encoding cre dependent expression of membrane mCherry

Depositor

Oscar Marin

Publication

Ciceri et al Nat Neurosci. 2013 Sep;16(9):1199-210. d

Insert

Mem-mCherry

Use

Retroviral

Promoter

CAG

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINK-UBE2S

Plasmid

#66714

Purpose

Expresses UBE2S (1-196) in E. coli

Depositor

David Komander

Publication

Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

Insert

e2-epf (UBE2S )

Tags

GST-3C

Expression

Bacterial

Promoter

T7

Available since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pUC-UAS1B8-TEF(272)

Plasmid

#44376

Depositor

Hal Alper

Publication

Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

Insert

UAS1B8-TEF(272) promoter

Use

Synthetic Biology

Expression

Bacterial

Mutation

N/A

Promoter

UAS1B8-TEF(272)

Available since

April 16, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

Anthony Bretscher, David Bryant

Publication

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (WT)

Plasmid

#182923

Purpose

Mammalian expression plasmid for human CBS

Depositor

Philip Eaton

Publication

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Gi-c010

Plasmid

#173080

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GAP

Tags

mCitrine

Expression

Bacterial

Mutation

mCitrine + last 10 residues of GNA1, codon optimi…

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MacroH2A1.1-c002

Plasmid

#173087

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

MACROH2A1 (MACROH2A1 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

Q162-N369, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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APLF-c001

Plasmid

#173077

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

APLF (APLF Human)

Tags

mCerulean

Expression

Bacterial

Mutation

K360-V448, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TARG1-c002

Plasmid

#173108

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

TARG1

Tags

mCerulean

Expression

Bacterial

Mutation

Full length protein

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUC119 T7 hTRmin

Plasmid

#127858

Purpose

T7 RNA Pol for in vitro transcription

Depositor

Kathleen Collins

Publication

Wu et al EMBO J. 2014 Apr 16;33(8):921-35. doi: 1

Insert

hTR (TERC Human)

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xHA-MKK4-EE

Plasmid

#47577

Purpose

Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4

Depositor

Kevin Janes

Publication

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Insert

MKK4-EE (MAP2K4 Human)

Tags

3xHA and GST

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pLIC-EcTOP

Plasmid

#224463

Purpose

Expression of recombinant Escherichia coli topoisomerase I

Depositor

Yuk-Ching Tse-Dinh

Publication

Tan et al Nucleic Acids Res. 2015 Dec 15;43(22):11

Insert

Escherichia coli TopA

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCAH01Sp::Cas9

Plasmid

#128160

Purpose

Ptet-controlled expression of S. pyogenes Cas9

Depositor

Calvin Henard

Publication

Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

Insert

Sp Cas9

Expression

Bacterial

Promoter

Ptet

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMJA289= pSin-EF2-TRAV10-P2A-TRBV25-Puro

Plasmid

#116876

Purpose

stable TCR expression in human T cells

Depositor

Marcos Alcocer

Publication

Wang et al Biotechnol Lett. 2019 Oct;41(10):1095-11

Insert

TRAV10 - P2A - TRBV25 (TRAV10, TRBV25-1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1-alpha

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pFCK(0.4)GW

Plasmid

#27229

Depositor

Pavel Osten

Publication

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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