We narrowed to 6,483 results for: poly

1. pCAG-T3-HypaCAS9-pA

   Plasmid

   #131467

   Purpose

   Expresses HypaCas9 nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Ikeda et al Commun Biol. 2019 Oct 10;2:371. doi: 10.

   Insert

   Codon optimized HypaCas9
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGS-BacA-21222-FLP-3xFLAG

   Plasmid

   #109165

   Purpose

   Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

   Insert

   Flp
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBac(3xP3-gTc’v; Valcyrie.LR)

   Plasmid

   #119066

   Purpose

   Valcyrie construct for CRISPR-mediated lox excision in Tribolium castaneum (high)

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2019 Jan 4;146(1). pii: dev

   Inserts

   pBacL
   Tc-U6a-Lox.L
   Tc-hsp68 Promoter
   NLS-Cas9
   Tc-hsp68 3'UTR
   Tc-U6B-Lox.R
   SV40polyA
   Tc-vermilion
   3xP3 TATA
   pBacR
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L TriCatcher-MMP

   Plasmid

   #112632

   Purpose

   Two SpyCatchers linked by an elastin-like polypeptide with an MMP-cleavable sequence and a C-terminal SnoopCatcher

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   TriCatcher-MMP
   

   Use

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Mutation

   central matrix metalloproteinase-cleavable sequen…

   Promoter

   T5

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3R112C/T180C-GG

   Plasmid

   #85093

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. p1G108C-GG

   Plasmid

   #85092

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 1
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Glycine 108 to Cysteine

   Promoter

   T7 promoter

   Available since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET15b+PCNA3 T180C

   Plasmid

   #66181

   Purpose

   Expresses the T180C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   T180C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b+PCNA3 R112C

   Plasmid

   #66180

   Purpose

   Expresses the R112C mutant of Sulfolobus solfataricus PCNA3

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 3
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   R112C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET15b+PCNA2 E105C

   Plasmid

   #66176

   Purpose

   Expresses the E105C mutant of Sulfolobus solfataricus PCNA2

   Depositor

   Teruyuki Nagamune

   Article

   Hirakawa et al Biotechnol Bioeng. 2013 Jul;110(7):1858-

   Insert

   DNA polymerase sliding clamp 2
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   E105C

   Promoter

   T7 promoter

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIB-HISX6-TEV-RSP4

    Plasmid

    #176693

    Purpose

    Expression of Chlamydomonas reinhardtii RSP4 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP4
    

    Use

    Tags

    HISX6

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLIB-RSP5

    Plasmid

    #176694

    Purpose

    Expression of Chlamydomonas reinhardtii RSP5 recombinant protein in insect cells

    Depositor

    Ron Vale

    Article

    Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

    Insert

    RSP5 (RSP5 Chlamydomonas reinhardtii)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    None

    Promoter

    polyhedrin

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFS462

    Plasmid

    #89065

    Purpose

    his7 integration plasmid with Z3EVpr:GFP

    Depositor

    Nick Rhind

    Article

    Ohira et al Yeast. 2017 Aug;34(8):323-334. doi: 10.1

    Insert

    Z3EV promoter GFP-NLS leu1 C-terminal 300 nt
    

    Use

    S. pombe his7 integration vector

    Tags

    Expression

    Mutation

    Promoter

    Z3EV

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLiLucB

    Plasmid

    #31596

    Depositor

    Michele Calos

    Article

    Woodard et al PLoS One. 2010 . 5(6):e11367.

    Insert

    alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEAST_J23101-amidase

    Plasmid

    #128135

    Purpose

    The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

    Depositor

    Jean-Loup Faulon

    Article

    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

    Insert

    amidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWN_U6-TRAC-miniGag-Cas9

    Plasmid

    #228959

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pWN_U6-B2M-miniGag-Cas9

    Plasmid

    #228958

    Purpose

    miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag-Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC0043-PspCas13b crRNA backbone

    Plasmid

    #103854

    Purpose

    For cloning of guide RNAs compatible with PspCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Has Service

    Cloning Grade DNA

    Insert

    U6-BbsI-BbsI-PspCas13b DR-polyT
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pVSVΔG-eGFP-linker

    Plasmid

    #231707

    Purpose

    Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

    Depositor

    Ron Geller

    Article

    Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

    Insert

    VSV antigenome
    

    Use

    Vsv expression

    Tags

    Expression

    Mammalian

    Mutation

    ΔG (VSV glycoprotein removed)

    Promoter

    T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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