We narrowed to 5,472 results for: KRAS

1. pHR-UCOE-SFFV-dCas9-KRAB(Kox1)-MeCP2-P2A-EGFP

   Plasmid

   #188902

   Purpose

   dCas9 with a C-term NLS-KRAB(Kox1)-MeCP2 fusion (no linker), and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-KRAB(Kox1)-MeCP2
   

   Use

   Lentiviral

   Tags

   NLS-KRAB(Kox1)-MeCP2 fusion (no linker) and P2A-G…

   Promoter

   SFFV

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

   Plasmid

   #84245

   Purpose

   Expresses direct fusion KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   KRAB-tagBFP-Sp dCas9
   Puro
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), HA tag, KRAB, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide3

   Plasmid

   #125430

   Purpose

   CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   July 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLV-hUV6-sgRNA-dCas9-KRAB-TagBFP2 (identifier AAAA-0244)

   Plasmid

   #202553

   Purpose

   Nontargeting control vector with TagBFP2

   Depositor

   Frederic Meunier

   Article

   Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:

   Insert

   Humanized dCas9-KRAB-TagBFP2 (tag blue fluorescent protein 2) (TRIM28 S. Pyogenes (dCas9), H. sapiens (KRAB), Entacmaea quadricolor (TagBFP2))
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2

   Mutation

   Puromycin-resistance gene in the pLV hU6-sgRNA hU…

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

   Plasmid

   #118169

   Purpose

   CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2

   Plasmid

   #118170

   Purpose

   CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

   Plasmid

   #159560

   Purpose

   E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)

   Depositor

   Dominic Esposito

   Article

   Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

   Insert

   His6-MBP-tev-Avi-Hs.KRAS4b(2-169) (KRAS Human)
   

   Tags

   His6-MBP-tev-Avi

   Expression

   Bacterial

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide3

   Plasmid

   #118171

   Purpose

   CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   KRAB-dCas9-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hPGK and U6

   Available Since

   April 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

   Plasmid

   #188765

   Purpose

   dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Depositor

   Jonathan Weissman, Marco Jost

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9-XTEN80-KRAB(Kox1)
   

   Use

   Lentiviral

   Tags

   HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

   Promoter

   SFFV

   Available Since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188772

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    CMV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188771

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    EFS

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R989-X28-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Plasmid

    #159548

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12D

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R989-X09-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

    Plasmid

    #159541

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) G12D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12D

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R989-X10-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

    Plasmid

    #159542

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) G12V (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G12V

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. R989-X11-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

    Plasmid

    #159543

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) G13D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G13D

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. R989-X13-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

    Plasmid

    #159545

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61R (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Q61R

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. R989-X31-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

    Plasmid

    #159551

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Q61L

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. R989-X12-566: His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

    Plasmid

    #159544

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-169) Q61L (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    Q61L

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

    Plasmid

    #159692

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L

    Depositor

    Dominic Esposito

    Article

    Procter et al Protein Expr Purif. 2018 Nov;151:99-105.

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    M188L

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. R989-X30-566: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Plasmid

    #159550

    Purpose

    E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Bacterial

    Mutation

    G13D

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge