We narrowed to 8,554 results for: sgRNA

1. TLCV2 - sgPTEN

   Plasmid

   #202759

   Purpose

   Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

   Depositor

   Christopher Counter

   Article

   Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

   Insert

   gRNA targeting PTEN
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW1310

   Plasmid

   #61253

   Purpose

   Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

   Depositor

   Jordan Ward

   Article

   Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

   Insert

   U6 promoter
   

   Use

   CRISPR; Cloning template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pyEvolvR (enCas9-PolI5M)

   Plasmid

   #158073

   Purpose

   Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber

   Article

   Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

   Insert

   enCas9-PolI5M
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. YN2_1_LT58_X2site

   Plasmid

   #177705

   Purpose

   Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDD143

   Plasmid

   #119880

   Purpose

   Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

   Depositor

   Tae Seok Moon

   Article

   DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

   Insert

   pBAD_dCas9sth1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

   Plasmid

   #159908

   Purpose

   Mutagenesis of Htr2a

   Depositor

   Larry Zweifel

   Insert

   Htr2a (Htr2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRN1120

   Plasmid

   #101750

   Purpose

   Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

   Depositor

   Rene Verwaal

   Article

   Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR8-attL5-pegRNA-attR3

   Plasmid

   #213054

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpyB

    Plasmid

    #220186

    Purpose

    sgRNA expression vector - pBG35 promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    pBG35 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBG35

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

11. PlayBack-U6-Xba1

    Plasmid

    #203300

    Purpose

    For expression of sgRNAs in a PlayBack Vector with XbaI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTJV1ScKm-rpoB

    Plasmid

    #166980

    Purpose

    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance

    Depositor

    Christopher Reisch

    Article

    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

    Insert

    NeoR/KanR
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. px330 SUZ12 - pDY083

    Plasmid

    #171109

    Purpose

    Expresses Cas9 and an sgRNA targeting SUZ12

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. sgPal7 (p2Tol-U6-sgPal7-HygR)

    Plasmid

    #71484

    Purpose

    Expresses palindromic sgRNA for use in self-cloning CRISPR

    Depositor

    Richard Sherwood

    Article

    Arbab et al Stem Cell Reports. 2015 Oct 27. pii: S22

    Insert

    sgPal7
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. px330 EZH2 - pDY052

    Plasmid

    #171107

    Purpose

    Expresses Cas9 and an sgRNA targeting EZH2

    Depositor

    Thomas Cech

    Article

    Youmans et al Genes Dev. 2018 Jun 1;32(11-12):794-805.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GEARBOCS-SPARCL1-GeneTRAP

    Plasmid

    #218184

    Purpose

    To KO and genetrap mouse Hevin simultaneously

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GEARBOCS-SPARCL1 KO

    Plasmid

    #218182

    Purpose

    To KO Hevin in astrocytes

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBC2103-reci

    Plasmid

    #117172

    Purpose

    U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    reci_arCas9 without NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAN-PA4-RFP

    Plasmid

    #62250

    Purpose

    reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

    Insert

    mRFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pA4

    Available since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge