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We narrowed to 18,759 results for: nls

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Showing: 11721 - 11760 of 18759 results

  1. HaloTag-LacI-KRAB

    Plasmid
    #232638
    Purpose
    lac repressor (LacI) which binds lacO sites; labeled with HaloTag and coupled with Krüppel-associated box (KRAB) repressor
    Depositor
    Karsten Rippe
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag-LacI-KRAB
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Feb. 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    232638-plasmid-map-sequence-id-462856

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  20. pABE-dGFP

    Plasmid
    #226863
    Purpose
    Constitutively expresses mCherry as a transfection marker. Expresses non-fluorescent EGFP, deactivated by A111V mutation, that can be reactivated by active adenine base editing
    Depositor
    Alexis Komor
    Publication
    Osgood et al Methods Enzymol. 2025;712:351-404. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP
    Expression
    Mammalian
    Mutation
    A111V
    Promoter
    CMV
    Available since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    226863-plasmid-map-sequence-id-450497

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  29. TRE3GV-SpdCas9-Dnmt3A/3L-V2

    Plasmid
    #222505
    Purpose
    Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to Dnmt3A/3L variant V2 (E814G) and hPGK promoter driven mCherry
    Depositor
    Isaac Hilton
    Publication
    Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021
    Inserts
    S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L variant V2 (E814G) engineered fusion
    mCherry
    Use
    Lentiviral
    Tags
    FLAG Tag
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9; E814G in Dnmt3A
    Promoter
    TRE3GV and hPGK
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    222505-plasmid-map-sequence-id-441223

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  30. pPB9v2

    Plasmid
    #210029
    Purpose
    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210029-plasmid-map-sequence-id-414001

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  32. pPB1163

    Plasmid
    #210032
    Purpose
    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210032-plasmid-map-sequence-id-413995

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  35. MOBE3

    Plasmid
    #219943
    Purpose
    Mammalian expression of MOBE3 protein (apt-CBE-SL3_PT2A_apt-ABE8e)
    Depositor
    Alexis Komor
    Publication
    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    apt-CBE-SL3_PT2A_apt-ABE8e
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    219943-plasmid-map-sequence-id-460114

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  39. pAV-H1promoter-2xTetO-High_ribosome_load-Tornado-2xALFA-10xSunTag-Xbp1(S255A)

    Plasmid
    #231595
    Purpose
    Express high ribosome loading circular RNA translation reporter 2xALFATag-10xSunTag-Xbp1(S255A) pause site under H1 promoter
    Depositor
    Marvin Tanenbaum
    Publication
    Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.
    Insert
    Kozak sequence-Tornado-2xALFATag-10xSunTag-Xbp1(S255A) pause sequence
    Expression
    Mammalian
    Available since
    Jan. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    231595-plasmid-map-sequence-id-459554

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  40. pCas9-EcRT-GAL-Kan

    Plasmid
    #232092
    Purpose
    Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.
    Depositor
    Jeffrey Lewis
    Publication
    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:
    Insert
    SpCas9 and Ec86 reverse transcriptase
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Ec86 reverse transcriptase is codon optimized for…
    Promoter
    GAL1-GAL10
    Available since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    232092-plasmid-map-sequence-id-459849

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Showing: 11721 - 11760 of 18759 results