We narrowed to 9,167 results for: Pol

1. pNSSCP

   Plasmid

   #183188

   Purpose

   PACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; DivIVA-sfGFP expressed under T

   Depositor

   Hsiao-Chun Huang

   Article

   Lin et al Nat Commun. 2021 Feb 9;12(1):888. doi: 1

   Inserts

   DivIVA and superfolder GFP fusion protein
   SpmX450 and T7 181+ fusion protein
   T7 RNAP 1-179 variant and SpmX450 fusion protein
   mRFP1 and popZ fusion protein
   

   Expression

   Bacterial

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2031

   Plasmid

   #49134

   Purpose

   AAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-Ple305-iCre
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   OLIG1

   Available Since

   May 10, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pD21-hgAPOAI

   Plasmid

   #124962

   Purpose

   Express ApoAI in the liver

   Depositor

   Kazuhiro Oka

   Article

   Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

   Insert

   human APOAI gene (LPA Human)
   

   Use

   Adenoviral

   Promoter

   human APOAI

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C3-PLK4-S305A-3xFLAG

   Plasmid

   #69839

   Purpose

   This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

   Depositor

   Michel Bornens

   Article

   Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

   Insert

   PLK4 (PLK4 Human)
   

   Tags

   3xFLAG tag and EGFP

   Expression

   Mammalian

   Mutation

   S305A

   Promoter

   CMV

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEE062

   Plasmid

   #176819

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   701
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST27-GST-ATXN1 FL(84Q)

   Plasmid

   #21754

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 84 Glutamines and no…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pEE077

   Plasmid

   #176834

   Purpose

   Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

   Depositor

   Gregg Beckham

   Article

   Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

   Insert

   716
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CAG-Brn2-DBD-EnR

   Plasmid

   #19714

   Depositor

   Connie Cepko

   Article

   Kim et al J Neurosci. 2008 Jul 30. 28(31):7748-64.

   Insert

   Fly engrailed 36240 (Pou3f2, en Fly, Mouse)
   

   Expression

   Mammalian

   Mutation

   mouse Brn2 DNA binding domain-Drosophila engraile…

   Available Since

   Oct. 27, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pT2/GD-IRES-GFP-CTNNB1

   Plasmid

   #192868

   Purpose

   Carries a Sleeping Beauty (SB) transposon vector that can be used to deliver an activated human CTNNB1(S33Y) transgene into cells, when a source of SB transposase is also co-introduced.

   Depositor

   David Largaespada

   Article

   Keng et al Hepatology. 2013 Jan;57(1):120-30. doi:

   Inserts

   luc+
   EGFP
   CTNNB1
   

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBactin-Tubg1-mNeonGreen

    Plasmid

    #196869

    Purpose

    Expression of the gamma-tubulin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Tubg1-mNeonGreen (Tubg1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEMS1977

    Plasmid

    #49109

    Purpose

    AAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple251-icre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    C8ORF46

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. HOXA9-MSCV-CD24

    Plasmid

    #20975

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2008 Jul . 28(14):4609-19

    Inserts

    HOXA9 (Hoxa9 Mouse)
    CD24 (CD24 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Full length cDNA with ~1.1kb 3'UTR with 2 pu…

    Available Since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. VIPR1

    Plasmid

    #51865

    Purpose

    Expresses full-length Vasoactive intestinal polypeptide receptor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    VIPR1 (VIPR1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDEST27-GST-ATXN1 FL(30Q)

    Plasmid

    #21753

    Depositor

    Huda Zoghbi

    Article

    Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

    Insert

    Ataxin-1 (ATXN1 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Mutation

    insert contains a stretch of 32 amino acids (30 Q…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pEMS1996

    Plasmid

    #49126

    Purpose

    AAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-Ple267-iCre
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    UGT8

    Available Since

    May 10, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLKO.1P DDX11_2

    Plasmid

    #160778

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_2
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1P DDX11_1

    Plasmid

    #160777

    Purpose

    Suppress DDX11

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shDDX11_1
    

    Use

    Lentiviral

    Available Since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET19-M(BRCT)TdTwt

    Plasmid

    #207459

    Purpose

    Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) variant, including the N-terminal BRCT domain

    Depositor

    Jay Keasling

    Article

    Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

    Insert

    M(BRCT)TdTwt (Dntt Mouse)
    

    Tags

    10xHis-MBP

    Expression

    Bacterial

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEMS2157

    Plasmid

    #70118

    Purpose

    Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.

    Depositor

    Elizabeth Simpson

    Article

    Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

    Insert

    ssAAV-MCS-EmGFP-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBetaActin-KIF13aMotor-mScarlet-FKBP

    Plasmid

    #191337

    Purpose

    Expresses the fluorescently labeled plus-end directed KIF13a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

    Depositor

    Frank Bradke

    Article

    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

    Insert

    KIF13aMotor-mScarlet-FKBP (KIF13A Human)
    

    Expression

    Mammalian

    Mutation

    motor domain aa 1-639

    Available Since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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