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YCe1911 HC_Kan_Insulator_FB_p2

Plasmid

#100686

Purpose

part designed to occupy position 22 of EMMA. Functional category: Insulator

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

Insulator_FB

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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YCe2052 HC_Kan_DmrC_p9

Plasmid

#100693

Purpose

part designed to occupy position 9 of EMMA. Functional category: Dimerization domain

Depositor

Yizhi Cai

Publication

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

DmrC

Use

Synthetic Biology

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-Akt2(K14A)

Plasmid

#86624

Purpose

Expresses mCherry-tagged human Akt2(K14A) membrane binding mutant

Depositor

Thomas Leonard, Ivan Yudushkin

Publication

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt2(K14A) (AKT2 )

Expression

Mammalian

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBN40

Plasmid

#86715

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Plmn-1::gfp::his-58

Expression

Worm

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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LI A-C 35S c2

Plasmid

#54409

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

cauliflower mosaic virus 35S promoter

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI A-B Ubi (+ intron) c4

Plasmid

#54408

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

L. japonicus polyubiquitin promoter

Use

Cloning vector

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pSuper 44-2

Plasmid

#51598

Purpose

shRNA 2 against rat Sema4D

Depositor

Suzanne Paradis

Publication

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

shRNA2

Use

RNAi

Promoter

U6

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
TAL-4GA1

Plasmid

#41504

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

4GA1

Use

Pcr cloning vector

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
TAL-3TA4

Plasmid

#41472

Depositor

Veit Hornung

Publication

Schmid-Burgk et al Nat Biotechnol. 2012 Dec 16. doi: 10.103

Insert

3TA4

Use

Pcr cloning vector

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pSMPPv2-Tau-RING

Plasmid

#233688

Purpose

Expression of Tau-RING protein in mammalian cells

Depositor

Leo James

Publication

Miller et al Cell. 2024 Oct 17;187(21):5967-5980.e17.

Insert

Tau protein fused to TRIM21 RING domain

Use

Lentiviral

Expression

Mammalian

Available since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pLPR2

Plasmid

#209980

Purpose

Contains Level 0 Part: resistance cassette (cat) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

resistance cassette (cat)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLPO5

Plasmid

#209987

Purpose

Contains Level 0* Part: replication origin (256rep) for the construction of Level 2 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

replication origin (256_rep)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pLPS2

Plasmid

#209972

Purpose

Contains Level 0 Part: ribosome binding site (L. plantarum optimized) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

RBS (Lp)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSpyB

Plasmid

#220186

Purpose

sgRNA expression vector - pBG35 promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG35 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG35

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only
AA183

Plasmid

#216025

Purpose

Fragmid fragment: (Cas protein) for prime editing; R221K;N394K for increased efficiency

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (H840A)_v2.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA028

Plasmid

#216008

Purpose

Fragmid fragment: (Cas protein) nickase Cas for base editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (D10A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA060

Plasmid

#216017

Purpose

Fragmid fragment: (Cas protein) nickase Cas for prime editing

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has service

Cloning Grade DNA

Insert

nCas9 (H840A)_v1.1 [Sp]

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-mRuby3

Plasmid

#214923

Purpose

expression vector control - contitutive expression of mRuby33 alone

Depositor

Ophir Shalem

Publication

Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

Insert

mRuby3

Use

Lentiviral

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pLP-sfgfp

Plasmid

#209994

Purpose

Contains Level 0 Part: Coding sequences (sfGFP) for the construction of Level 1 plasmids

Depositor

Georg Fritz, Thorsten Mascher

Publication

Stukenberg et al ACS Synth Biol. 2021 Aug 20;10(8):1904-1

Insert

sfGFP

Expression

Bacterial

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pL0_CD-greenlantern

Plasmid

#202141

Purpose

Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Green lantern gene

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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