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We narrowed to 18,759 results for: nls

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Showing: 11801 - 11840 of 18759 results

  3. pAGK112

    Plasmid
    #228127
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    228127-plasmid-map-sequence-id-455412

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  4. pAGK61

    Plasmid
    #228121
    Purpose
    Express node 1467 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1467, split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228121-plasmid-map-sequence-id-455410

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  8. pDIV300

    Plasmid
    #204968
    Purpose
    AMA1 plasmid with Aspergillus optimized Mad7 and hph (hygromycin) resistance marker
    Depositor
    Uffe Mortensen
    Publication
    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do
    Inserts
    Mad7
    hph (hygromycin resistance marker)
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Promoter
    Aspergillus nidulans tef1 promoter and Aspergillu…
    Available since
    Nov. 22, 2024
    Availability
    Academic Institutions and Nonprofits only
    204968-plasmid-map-sequence-id-454124

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  10. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

    Plasmid
    #228722
    Purpose
    Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228722-plasmid-map-sequence-id-454017

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  12. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid
    #228723
    Purpose
    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2 nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228723-plasmid-map-sequence-id-454010

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  13. pEJS2696-CMV-Nme2SmuCas9-D56R

    Plasmid
    #228717
    Purpose
    Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nme2SmuCas9-D56R nuclease
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D56R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228717-plasmid-map-sequence-id-454003

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  14. pEJS2703-CMV-iNme2Smu-ABE8e-nt

    Plasmid
    #228724
    Purpose
    Expresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N-terminal ABE8e with iNme2Smu nCas9 (D16A)
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228724-plasmid-map-sequence-id-454004

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  15. pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R

    Plasmid
    #228732
    Purpose
    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D56R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228732-plasmid-map-sequence-id-453998

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  16. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

    Plasmid
    #228735
    Purpose
    Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228735-plasmid-map-sequence-id-453996

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  17. pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R

    Plasmid
    #228731
    Purpose
    Expresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A E932R
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228731-plasmid-map-sequence-id-453993

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  18. pEJS2713-CMV-iNme2-ABE8e-i1-L10

    Plasmid
    #228734
    Purpose
    Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells
    Depositor
    Erik Sontheimer
    Publication
    Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Domain inlaid iNme2 nCas9 (D16A)-i1-L10
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    228734-plasmid-map-sequence-id-453992

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  20. TadA-8e-eIscBn-C-HMG-D

    Plasmid
    #227145
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoter
    Depositor
    Dali Li
    Publication
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227145-plasmid-map-sequence-id-453986

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  21. TadA-8e-eIscBn

    Plasmid
    #227018
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e driven by CMV promoter
    Depositor
    Dali Li
    Publication
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227018-plasmid-map-sequence-id-453983

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  22. TadA-8e-eIscBn-N-HMG-D

    Plasmid
    #227143
    Purpose
    Vector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoter
    Depositor
    Dali Li
    Publication
    Xue et al Mol Cell. 2024 Aug 22;84(16):3128-3140.e
    Insert
    bpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
    Mutation
    D96R/E84R/V159R/H339A
    Promoter
    CMV
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    227143-plasmid-map-sequence-id-453972

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  24. pX330-PITCh-MYO1C

    Plasmid
    #227305
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227305-plasmid-map-sequence-id-452213

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  29. pEC1.2/EC1.1_Cas9i_TagRFP

    Plasmid
    #227249
    Purpose
    CRISPR plasmid with Cas9i expressed under egg cell promoter - TagRFP seed selection
    Depositor
    Christian Fankhauser
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Promoter
    pEC1.2/EC1.1
    Available since
    Nov. 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    227249-plasmid-map-sequence-id-451525

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  30. pX330-PITCh-MAPRE1

    Plasmid
    #207793
    Purpose
    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in.
    Depositor
    Laurence Pelletier
    Publication
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA Targeting C-terminus of MAPRE1 (MAPRE1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    207793-plasmid-map-sequence-id-451404

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  33. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Publication
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

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  34. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Publication
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

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  37. TadA-NG-CBE

    Plasmid
    #227420
    Purpose
    C-to-T base editing in mammalian cells
    Depositor
    Yang Sun
    Publication
    Liu et al Adv Sci (Weinh). 2024 Sep 19:e2405628. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA-nSpCas9-NG-2xUGI (AICDA Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227420-plasmid-map-sequence-id-450076

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Showing: 11801 - 11840 of 18759 results