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pETM30-C9ORF78

Plasmid

#193367

Purpose

Expression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78 (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGAL1-VP1 (LEU2)

Plasmid

#182480

Purpose

Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Insert

deltaVP1

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-NODALvar-matMYC

Plasmid

#115641

Purpose

For targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycycline

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

TRE3G

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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NODALvar-matMYC

Plasmid

#115249

Purpose

For mammalian expression of a human NODAL splice variant open reading frame with an internal MYC tag

Depositor

Lynne Postovit

Article

Findlay et al (2018) bioRxiv 276170

Insert

NODAL splice variant (NODAL Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-P110A

Plasmid

#180237

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domain of ColE1 …

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pTH32 [pmyo-3::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168168

Purpose

Expression of YFP-CaCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTH42 [punc-17::YFP-CaCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168171

Purpose

Expression of YFP-CaCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-CaCyclOp(A-2x)

Tags

YFP

Expression

Worm

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M5

Plasmid

#106325

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site insertion mutant EEKQNA-->EEK…

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3.1-OPNc M4

Plasmid

#106324

Purpose

Mammalian expression of mutant osteopontin

Depositor

Georg Weber

Article

Shen et al Mol Carcinog. 2014 Jun;53(6):480-7. doi:

Insert

OPNc (SPP1 Human)

Expression

Mammalian

Mutation

OPNc splice site deletion mutant EEKQNA-->EEKNA

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pSABAD518

Plasmid

#46374

Depositor

Hideo Iwai

Article

Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

Insert

TvoVMA intein, inactive

Tags

H6-GB1 and SH3

Expression

Bacterial

Mutation

N186A, T+1A mutations, inactive

Promoter

pBAD

Available Since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-479.E152Q

Plasmid

#17518

Depositor

David McKay

Article

Wang et al Biophys J. 2008 Jan 1. 94(1):L01-3.

Insert

B. subtilis DEAD-box helicase YxiN

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

E152Q mutation

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only
p414GPD-3xFLAG-Nop4 L306P

Plasmid

#169262

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 L306P (NOP4 Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu306Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-RBM28 L351P

Plasmid

#169266

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 L351P (RBM28 Human)

Tags

3xFLAG

Expression

Yeast

Mutation

p.(Leu351Pro)

Promoter

GPD

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E8 WT minigene

Plasmid

#169271

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E8 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-human Cyclin L1 alpha-IRES-EGFP

Plasmid

#240140

Purpose

Express Cyclin L1 alpha

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 alpha (CCNL1 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFLEX-FLAG-human Cyclin L1 alpha

Plasmid

#240145

Purpose

Express Cyclin L1 alpha

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 alpha (CCNL1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SRalpha

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-human Cyclin L1 gamma-IRES-EYFP

Plasmid

#240143

Purpose

Express Cyclin L1 gamma

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 gamma (CCNL1 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pMSCV-HA-human Cyclin L1 beta-IRES-EGFP

Plasmid

#240141

Purpose

Express Cyclin L1 beta

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 beta (CCNL1 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pFLEX-FLAG-human Cyclin L1 beta

Plasmid

#240147

Purpose

Express Cyclin L1 beta

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L1 beta (CCNL1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

SRalpha

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TNT4-Zfr-P2A-HA-luciferase

Plasmid

#223188

Purpose

Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

Depositor

Yuxuan Guo

Article

Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

Insert

luciferase (LOC116160065 firefly)

Use

AAV

Tags

HA and engineered P2A

Promoter

TNNT2

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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