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Plasmid#225455PurposeExpress mEGFP-fusion protein of FXR1 isoform a (N202P)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationN202PPromoterCMVAvailable sinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CP4
Plasmid#223336PurposeMapping of cytosol-facing chloroplast outer membrane proximity proteome by proximity-dependent biotinylation in living Arabidopsis cellsDepositorInsertToc64-EGFP-miniTurboID-3HA
UseTagsEGFP-miniTurboID-3HAExpressionPlantMutationPromoterCaMV35SAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3puro-mGFP-FXR1a-Ccswap
Plasmid#225460PurposeExpress EGFP-fusion protein of FXR1 isoform a (CCswap)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationCCswap (swapped positions of CC2 and CC1)PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC1-CC1
Plasmid#225458PurposeExpress EGFP-fusion protein of FXR1 isoform a (CC1-CC1)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsEGFPExpressionMammalianMutationCC2 replaced with a second copy of CC1PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1-N1-KH1mut (T236D H237D)
Plasmid#225453PurposeExpress mEGFP-fusion protein of FXR1 isoform a (T236D H237D)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationT236D H237DPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)
Plasmid#225456PurposeExpress mEGFP-fusion protein of FXR1 isoform a (V361P)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationV361PPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)
Plasmid#225466PurposeExpress mEGFP-fusion protein of FMR1 isoform 1 (V427P)DepositorInsertFMR1 isoform 1 (FMR1 Human)
UseTagsmEGFPExpressionMammalianMutationV427PPromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3puro-mGFP-FXR1a-CC2-CC2
Plasmid#225459PurposeExpress mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)DepositorInsertFXR1 isoform a (FXR1 Human)
UseTagsmEGFPExpressionMammalianMutationCC1 replaced with a second copy of CC2PromoterCMVAvailable sinceOct. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4636
Plasmid#215264PurposeModule for the expression of HISPS under the ed light regulated Etr8:CMVmin promoter and H3H under the copper inducible CBS:mDFR promoter and the constitutive expression of LUZ, CPH,EGFP and p19DepositorInsertEtr4:mDFR:HISPS-CBS4x:mDFR:H3HSF-LUZ-CPH-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB4541
Plasmid#215260PurposeModule for the expression of the N. nambi HISPS gene under the regulation of the Red light-regulated Etr4:mDFR promoter and TNOS in and the constitutve expression of CPH, LUZ, H3H, EGFP and p19.DepositorInsertEtr4:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFPEasy9R
Plasmid#218291PurposePlasmid-free in-situ promoter cloning and characterisation system in S. cerevisiae, allows the characterisation of a bidirectional promoter using yEGFP and E2-Crimson as reporters.DepositorInsertpURA3>KlURA3>tKlURA3-tPGK1KanMX>tAgTEF-pMAL32>MazF(RPL28i)-E2Crimson>tPDC1-pDDI2>ISceI>tSynth3>ura3(4,191)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxAmp1
Plasmid#218285PurposeAmplifying the gene copy number of heterologous genes (yEGFP and RelB) through ToxAmp (toxin-antitoxin-driven gene amplification) mechanismDepositorInsertHO(-253, -1)-pRPL8B>RelB>tPDC1-pTEF1>yEGFP>tURA3-ARS712-HO(-731, -264)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4QE2Crimson
Plasmid#218280PurposeIntroduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoterDepositorInsertpURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ExSYTE.H64A
Plasmid#216262Purposeinactive ExSYTE.H64A (P2A) EGFP with 3'UTR of Rat Arc DTE under synapsin promoterDepositorInsertExSYTE P2A EGFP - Rat DTE
UseAAVTagsExpressionMutationH64A mutation in each oTetRPromoterAvailable sinceMay 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTF-FLAG
Plasmid#217686PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2516PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC2 CTF-FLAG
Plasmid#217685PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR2 (CELSR2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2356PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1318
Plasmid#217960PurposeExpression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationL63A, F66A, I71APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1317
Plasmid#217959PurposeExpression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H4 (H4C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between V65 and F66PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1315
Plasmid#217957PurposeExpression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationF84A, L92A, Y99APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG
Plasmid#217719PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR3 (CELSR3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2522PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRC2 CTFdeltaTA-FLAG
Plasmid#217718PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertCELSR2 (CELSR2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-2362PromoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lnig-INTR-GFP
Plasmid#208904Purposeencodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFPDepositorInsertINTR
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBbleo-ErbB1
Plasmid#197359PurposeEncoding human ErbB1.DepositorInsertEGFR (EGFR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPgiCas13b-gRNA-1
Plasmid#184834PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PgiCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-1
Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only